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Detection of Mycoplasma wenyonii in cattle and transmission vectors by the loop-mediated isothermal amplification (LAMP) assay

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A Publisher’s Erratum to this article was published on 22 February 2014

Abstract

Mycoplasma wenyonii is a wall-less hemotrophic prokaryote with worldwide distribution. This paper describes the development of a LAMP method targeting 16S rRNA for specific detection of M. wenyonii in its vectors and cattle. The LAMP method is specific for M. wenyonii detection and more sensitive than PCR. A total of 330 blood samples from cattle were tested by LAMP and PCR detection, 71 (21.5 %) samples were positive by the LAMP, while only 62 (18.8 %) were positive by PCR. For detecting transmission vectors, 26 lice, 30 flies, and 26 mosquitoes were collected and 18 lice, 20 flies, and 21 mosquitoes were tested positive by LAMP and PCR. These results indicate that the LAMP assay is a simple and convenient diagnostic tool for M. wenyonii detection and can be used in epidemiological surveys.

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Acknowledgments

This study was in part supported by Special Fund for Agro-scientific Research in the Public Interest (200903036) from Ministry of Agriculture, People’s Republic of China. Dr. Liu Guang-yuan from Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences was thanked for providing the Theileria annulata DNA. The authors would like to thank Prof. Min Hu (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, China) for correction of this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Qiqi Song, Lixia Wang, Rui Fang, Muhammad Kasib Khan & Junlong Zhao

  2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Qiqi Song, Lixia Wang, Rui Fang, Muhammad Kasib Khan, Yanqin Zhou & Junlong Zhao

  3. Key Laboratory of Animal Epidemical Disease and Infectious Zoonoses, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Qiqi Song, Rui Fang, Yanqin Zhou & Junlong Zhao

  4. Hubei Provincial Center for Diseases Control and Prevention, Wuhan, Hubei, 430079, People’s Republic of China

    Lixia Wang

Authors
  1. Qiqi Song
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  2. Lixia Wang
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  3. Rui Fang
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  4. Muhammad Kasib Khan
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  5. Yanqin Zhou
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  6. Junlong Zhao
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Corresponding author

Correspondence to Junlong Zhao.

Additional information

Lixia Wang gave the same contribution as the first author.

An erratum to this article is available at http://dx.doi.org/10.1007/s11250-014-0555-z.

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Song, Q., Wang, L., Fang, R. et al. Detection of Mycoplasma wenyonii in cattle and transmission vectors by the loop-mediated isothermal amplification (LAMP) assay. Trop Anim Health Prod 45, 247–250 (2012). https://doi.org/10.1007/s11250-012-0197-y

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  • DOI: https://doi.org/10.1007/s11250-012-0197-y

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