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Effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating Murrah (Bubalus bubalis) buffaloes


The study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating Murrah buffaloes (Bubalus bubalis). Fifteen multiparous buffaloes (2–4 lactation) of early to mid lactation were divided in three homogenous groups T1 (control), T2, and T3 of five each. The animals in T1 were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group T2 and T3 were fed with same ration supplemented with 0.7 % (100 g/day) and 1.4 % (200 g/day) bypass fat (on dry matter intake (DMI) basis), respectively. The feed intake, milk yield, and milk composition were not influenced by supplemental bypass fat. However, fat-corrected milk (6.5 %) yield was higher (P < 0.05) in T3 (14.21) than that of T1 (9.83) and similar with T2 (11.05). Feed efficiency (milk yield/kg DMI) was higher (P < 0.05) in group T3 (0.51) than that of T1 (0.38) and T2 (0.41) indicating that buffaloes fed with bypass fat which is 1.4 % (200 g/day) of the diet were economically more efficient. The serum cholesterol level was higher (P < 0.01) in bypass fat-supplemented group (T2 and T3) of animals. Serum high density lipoprotein (HDL) cholesterol (good cholesterol) level was more (P < 0.05) than LDL cholesterol (bad cholesterol) level with higher dose of bypass fat in T3 than T2. It was concluded that bypass fat supplementation with 1.4 % of the diet (200 g/day) increased the fat-corrected milk production and feed efficiency along with serum HDL cholesterol level in lactating Murrah buffaloes.

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Fig. 1





Dry matter intake


Fat-corrected milk yield


High density lipoprotein


Low density lipoprotein

T1 :

Basal diet + no bypass fat (control)

T2 :

Basal diet + 0.7 % (100 g/day) bypass fat

T3 :

Basal diet + 1.4 % (200 g/day) bypass fat


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The authors are thankful to Kemin Industries South Asia Pvt. Ltd., India for providing EnerFAT (rumen protected fat) which was used for conducting the research trial.

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Correspondence to Biswanath Sahoo.

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Ranjan, A., Sahoo, B., Singh, V.K. et al. Effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating Murrah (Bubalus bubalis) buffaloes. Trop Anim Health Prod 44, 1615–1621 (2012).

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