Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India
- 350 Downloads
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CL), a chronic debilitating disease of goats. In the present study, a total of 575 goats of Sirohi breed on an organized farm situated in the semi-arid tropical region of Rajasthan, India were clinically examined. Pus samples from superficial lymph nodes of 27 (4.7%) adult goats presenting clinical lesions suggestive of CL were collected for bacteriological and molecular analyses. Of these goats, 51.9% yielded C. pseudotuberculosis on the basis of morphological, cultural and biochemical characteristics. A polymerase chain reaction (PCR) assay targeting proline iminopeptidase gene specific to C. pseudotuberculosis was developed that confirmed all 14 bacterial isolates. The specificity of the PCR product was confirmed by sequencing of the 551-bp amplicon in both senses, showing 98–100% homology with published sequences. Thus, overall prevalence rate based on clinical, bacterial culture and PCR assay were found to be 4.7%, 2.4% and 2.4%, respectively. The PCR assay developed in this study was found to be specific and rapid, and could be used for confirmation of CL in goats as an alternative method to generally cumbersome, time-consuming and less reliable conventional methods.
KeywordsCaseous lymphadenitis Corynebacterium pseudotuberculosis Proline iminopeptidase Polymerase chain reaction Lymph nodes Goat
We wish to thank the director of the Institute and Incharge, Livestock Farm Section for providing necessary facility and support. The work has been conducted in the Veterinary Type Culture project.
- D'Afonseca, V., Prosdocimi, F., Dorella, F.A., Pacheco, L. G. C., Moraes, P. M., Pena, I., Ortega, J. M., Teixeira, S., Oliveira, S. C., Coser, E. M., Oliveira, L. M., Oliveira, G. C., Meyer, R., Miyoshi, A. and Azevedo, V., 2010. Survey of genome organization and gene content of Corynebacterium pseudotuberculosis. Microbiology Research, 165, 312–320.CrossRefGoogle Scholar
- FAOSTAT 2011. Food and Agricultural organization, United Nations. http://faostat.fao.org/site/339/default.aspx>.
- Ghanbarpour, R. and Khaleghiyan, M., 2005. A study on caseous lymphadenitis in goats. Indian Veterinary Journal, 82, 1013–1014.Google Scholar
- Guimaraes, A.S., Carmo, F.B., Pauletti, R.B., Seyffert, N., Ribeiro, D., Lage, A.P., Heinemann, M.B., Miyoshi, A., Azevedo, V. and Gouveia, A.M.G., 2011. Caseous lymphadenitis: epidemiology, diagnosis, and control. Institute of Integrative Omics and Applied Biotechnology Journal, 2 (2), 33-43.Google Scholar
- Hassan, N. A., Al-Humiany, A.A., Bahobail, A.S. and Mansour, A.M.A., 2011. Bacteriological and pathological studies on caseous lymphadenitis in sheep in Saudi Arabia. International Journal of Microbiological Research, 2 (1), 28-37.Google Scholar
- Mubarak, M., Bastawrows, A.F., Abdel-Hafeez, M.M. and Ali, M.M., 1999. Caseous lymphadenitis of sheep and goats in Assiut farms and abattoirs. Assiut Veterinary Medicine Journal, 42, 89–112.Google Scholar
- Pacheco, L. G. C., Pena, R. R., Castro, T. L. P., Dorella, F. A., Bahia, R. C., Carminati, R., Frota, M. N. L., Oliveira, S. C., Meyer, R., Alves, F. S. F., Miyoshi, A. and Azevedo, V., 2007. Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. Journal of Medical Microbiology, 56, 480-486.PubMedCrossRefGoogle Scholar
- Pavan, M.E., Robles, C., Cairo, F.M., Marcellino, R. and Pettinari, M.J., 2011. Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using RNA polymerase β-subunit gene (rpo B). Research in Veterinary Science. doi:10.1016/j.rvsc.2011.02.2007.
- Quinn, P. J., Markey, B. K., Leonard, F. C., Hartigan, P., Fanning, S. and FitzPatrick, E. S., 2011. Veterinary microbiology and microbial disease, 2nd edition, (Wiley- Blackwell, West Sussex,UK )Google Scholar
- Zavoshti, F.R., Khoojine, A.B.S., Helan, J.S., Hassanzadeh, B. and Heydari, A.A., 2010. Frequency of caseous lymphadenitis (CLA) in sheep slaughtered in an abattoir in Tabriz: comparison of bacterial culture and pathological study. Comparative Clinical Pathology, doi: 10.1007/s00580-010-1154-7.