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Tropical Animal Health and Production

, Volume 44, Issue 7, pp 1319–1322 | Cite as

Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India

  • Jyoti Kumar
  • Fateh Singh
  • Bhupendra Nath TripathiEmail author
  • Rajiv Kumar
  • Shivendra Kumar Dixit
  • Ganesh Gangaram Sonawane
Brief Communication

Abstract

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CL), a chronic debilitating disease of goats. In the present study, a total of 575 goats of Sirohi breed on an organized farm situated in the semi-arid tropical region of Rajasthan, India were clinically examined. Pus samples from superficial lymph nodes of 27 (4.7%) adult goats presenting clinical lesions suggestive of CL were collected for bacteriological and molecular analyses. Of these goats, 51.9% yielded C. pseudotuberculosis on the basis of morphological, cultural and biochemical characteristics. A polymerase chain reaction (PCR) assay targeting proline iminopeptidase gene specific to C. pseudotuberculosis was developed that confirmed all 14 bacterial isolates. The specificity of the PCR product was confirmed by sequencing of the 551-bp amplicon in both senses, showing 98–100% homology with published sequences. Thus, overall prevalence rate based on clinical, bacterial culture and PCR assay were found to be 4.7%, 2.4% and 2.4%, respectively. The PCR assay developed in this study was found to be specific and rapid, and could be used for confirmation of CL in goats as an alternative method to generally cumbersome, time-consuming and less reliable conventional methods.

Keywords

Caseous lymphadenitis Corynebacterium pseudotuberculosis Proline iminopeptidase Polymerase chain reaction Lymph nodes Goat 

Notes

Acknowledgements

We wish to thank the director of the Institute and Incharge, Livestock Farm Section for providing necessary facility and support. The work has been conducted in the Veterinary Type Culture project.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jyoti Kumar
    • 1
  • Fateh Singh
    • 1
  • Bhupendra Nath Tripathi
    • 1
    Email author
  • Rajiv Kumar
    • 2
  • Shivendra Kumar Dixit
    • 1
  • Ganesh Gangaram Sonawane
    • 1
  1. 1.Division of Animal HealthCentral Sheep and Wool Research InstituteAvikanagarIndia
  2. 2.Animal Biotechnology SectionCentral Sheep and Wool Research InstituteAvikanagarIndia

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