Tropical Animal Health and Production

, Volume 44, Issue 7, pp 1323–1328 | Cite as

Growth performance in heavy lambs experimentally treated with 17 ß-estradiol

  • Guido Ruggero Loria
  • Roberto PuleioEmail author
  • Maria Rita Schiavo
  • Paolo Giambruno
  • Elena Bozzetta
  • Massimo Todaro
Brief Communication


European and Italian legislation have banned use of growth promoters in livestock since 1988, but epidemiological data show that anabolic drugs are still being used illegally. Recent surveys carried out on the cattle farms in Northern Italy have confirmed the presence of growth-promoting hormones. Authors report data on growth performances in 80 Valle del Belice × Comisana weaned lambs experimentally treated with 17 beta-estradiol with 0.5 ml solution of oil Depot Estradiol ® (containing 5 mg of 17β-estradiol valerate) by intramuscular injection into the thigh. The experiment was founded by the National Ministry of Health, to validate histological test for surveillance and control of growth-promoting hormones in sheep. This study confirmed the strong correlation between clinical and anatomopathological features and growth performances of treated animals. Otherwise, no significant differences were found on in vivo performance of the lambs. Estradiol treatment showed heavier shoulders and necks on treated lambs, while the loins were significantly lighter. Moreover, lamb-estradiol-treated groups showed lower separable and inseparable fat percentage than lamb-control groups.


Growth hormone 17 Beta-estradiol Growth performance 



This research was founded by the Italian Ministry of Health (Projects RF-IZP-2006-364645).


  1. Association of Official Analytical Chemists (AOAC). 2003. Official Methods of Analysis, 17th edition, revision 2, Gaithersburg, MD, USA.Google Scholar
  2. Barone R., 2003. Anatomia comparata dei mammiferi domestici. (Edagricole, Bologna).Google Scholar
  3. Bonanno A., Di Miceli G., Di Grigoli A., Frenda A. S., Tornambè G., Giambalvo D., Amato G., 2011. Effects of feeding green forage of sulla (Hedysarium coronarium L.) on lamb growth and carcass and meat quality. Animal, 5 (1),148–154PubMedCrossRefGoogle Scholar
  4. Carson A.F., Elliott C.T., Mackie D.P., McCaughey W.J., 2001. Active immunization of lambs with a monoclonal antibody against clenbuterol. Livestock Production Science, 68, 87–91.CrossRefGoogle Scholar
  5. Courtheyn D., Le Bizec B., Brambilla G., De Brabander H. F., Cobbaert E., Van de Wiele M., Vercammen J. and De Wasch K., 2002. Recent developments in the use and abuse of growth promoters. Analytica Chimica Acta, 473, 71–82.CrossRefGoogle Scholar
  6. ERSAF, 2008. Il mercato delle carni, produzione e consumo.
  7. Fisher A.V., De Boer H., 1994. The EAAP standard method of sheep carcass assessment. Carcass measurements and dissection procedures. Report of the EAAP Working Group on Carcass Evaluation, in cooperation with the CIHEAM Instituto Agronomico Mediterraneo of Zaragoza and the CEC Directorate General for Agriculture in Brussels. Livestock Production Science. 38,149–159CrossRefGoogle Scholar
  8. Groot MJ., 2006. Effects of phyto-oestrogens on veal calf prostate histology. Veterinary Research Communication;30(6),587–98CrossRefGoogle Scholar
  9. Laviolette L.A., Garson K., Macdonald E.A, Senterman M.K., Courville K., Crane C.A. and Vanderhyden B.C., 2010. 17ß-estradiol accelerates tumor onset and decreases survival in a transgenic mouse model of ovarian cancer. Endocrinology, 151, 3, 929–938.PubMedCrossRefGoogle Scholar
  10. Meyer HH, 2001. Biochemistry and physiology of anabolic hormones used for improvement of meat production. APMIS.109(1),1–8.PubMedCrossRefGoogle Scholar
  11. Ministry of Health, 2009. D.G. della Sicurezza degli Alimenti e della Nutrizione, Ufficio III. Relazione finale Piano Nazionale Residui: 16–
  12. Newbold R.R., Liehr J.G., 2000. Induction of uterine adenocarcinoma in CD-1 mice by catechol estrogens. Cancer Research. 15;60(2): 235–237.PubMedGoogle Scholar
  13. Pace V., Carbone K., Spirito F., Iacurto M., Terzano M.G., Verna M., Vincenti F., Settineri D., 2006. The effects of subterranean clover phytoestrogens on sheep growth, reproduction and carcass characteristics. Meat Science. 74, 616–622PubMedCrossRefGoogle Scholar
  14. Pezzolato M., Maurella C., Varello K., Meloni D., Bellino C., Borlatto L., Di Corcia D., Capra P., Caramelli M., Bozzetta E., 2011. High sensitivity of a histological method in the detection of low-dosage illicit treatment with 17b-estradiol in male calves. Food Control. 22,1668–1673.CrossRefGoogle Scholar
  15. Preziuso, G., Russo, C., Casarosa, L., Campodoni, G., Piloni, S., Cianci, D., 1999. Effect of diet energy source on weight gain and carcass characteristics of lambs. Small Ruminant Research. 33, 9–15.CrossRefGoogle Scholar
  16. Reig, Milagro; Toldrá, Fidel, 2009. Safety of meat and processed meat, food microbiology and food Safety. Springer, New York.Google Scholar
  17. SAS, 2004. Statistical Analysis System. SAS Institute Inc. Version 9.1.2, Cary, NC, USA.Google Scholar
  18. Scarth J, Akre C, van Ginkel L, Le Bizec B, De Brabander H, Korth W, Points J, Teale P, Kay J., 2009. Presence and metabolism of endogenous androgenic–anabolic steroid hormones in meat-producing animals: a review. Food Additive Contaminants: Part A. Vol.26(5),640–671.CrossRefGoogle Scholar
  19. Scintu M.F., Piredda G., 2007. Typicity and biodiversity of goat and sheep milk products. Small Ruminant Research, 68,221–231.CrossRefGoogle Scholar
  20. The European Parliament and the Council of the European Union, 2008. Directive 2008/97/EC of the European Parliament and the Council of 19 November 2008 amending Council Directive 96/22/EC concerning the prohibition on the use in stock farming of certain substances having a hormonal or thyrostatic action and of beta-agonists. Official Journal of the European Union, L 318, 9–1.Google Scholar
  21. Yu FL., 2002. 17 Beta-estradiol epoxidation as the molecular basis for breast cancer initiation and prevention. Asia Pacific Journal of Clinical Nutrition;11 Suppl 7, 460–466.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Guido Ruggero Loria
    • 1
  • Roberto Puleio
    • 1
    Email author
  • Maria Rita Schiavo
    • 1
  • Paolo Giambruno
    • 2
  • Elena Bozzetta
    • 3
  • Massimo Todaro
    • 4
  1. 1.Istituto Zooprofilattico Sperimentale of SicilyLaboratory of Histology and ImmunohistochemistryPalermoItaly
  2. 2.Department of Veterinary Public HealthPalermoItaly
  3. 3.Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’AostaTorinoItaly
  4. 4.Dipartimento DemetraUniversità degli Studi di PalermoPalermoItaly

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