Tropical Animal Health and Production

, Volume 44, Issue 7, pp 1451–1458 | Cite as

Serological and bacteriological responses of water buffalo (Bubalus bubalis) vaccinated with two doses of Brucella abortus strain RB51 vaccine

  • Anil Ramnanan
  • Michael Diptee
  • Zinora Asgarali
  • Mervyn Campbell
  • Abiodun Adewale AdesiyunEmail author
Original Research


Thirty-two water buffalo (Bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to Brucella abortus strain RB51 (RB51) vaccination in a dose–response study and to compare the use of two selective media for the isolation of RB51. The animals were randomly divided into three treatment groups. Groups I–III received the recommended vaccine dose (RD) twice 4 weeks apart, RD twice 18 weeks apart and saline once, respectively. Lymph nodes were excised from the three groups and subjected to bacteriological examination to determine the frequency of detection of RB51. Pre- and post-vaccination blood samples were collected and tested for B. abortus antibodies using the buffered plate agglutination test (BPAT), complement fixation test (CFT), and dot-blot assay. Sera taken at all post-inoculation weeks (PIW) were negative for field strain B. abortus using the BPAT. Antibody responses to RB51 were demonstrated in all vaccinates but not in controls by CFT and dot-blot assay from 1 PIW up to 16 weeks following booster vaccination. The agreement for both assays was 80.7% and there was a linear interdependence with a Pearson’s correlation coefficient value of 0.578. The frequency of isolation of RB51 from the two selective media used was not significantly different (P > 0.05).


Brucella abortus RB51 Vaccination Water buffalo 



Many individuals and organizations contributed significantly to this study. Agostini Farms in Icacos was very accommodating in providing the majority of study animals. Mora Valley, Caroni (1975) Limited housed and fed the experimental animals throughout the study. Laboratory technicians at the Veterinary Diagnostic Laboratory facilitated the use of equipment when necessary. Special thanks to Dr. R. Adone for performing the CFT on the sera. Coworkers at the Ministry of Agriculture, Land and Marine Resources (MALMR) willingly assisted in the collection of samples and restraint of animals. Our gratitude goes to The University of the West Indies in their recognition of the national importance to the funding of this project.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Anil Ramnanan
    • 1
  • Michael Diptee
    • 2
  • Zinora Asgarali
    • 2
  • Mervyn Campbell
    • 2
  • Abiodun Adewale Adesiyun
    • 2
    Email author
  1. 1.Ministry of Agriculture, Lands and Marine ResourcesPort of SpainTrinidad and Tobago
  2. 2.School of Veterinary Medicine, Faculty of Medical SciencesUniversity of the West IndiesSt. AugustineTrinidad and Tobago

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