Tropical Animal Health and Production

, Volume 44, Issue 7, pp 1417–1421 | Cite as

Detection of antibodies and risk factors for infection with bovine respiratory syncytial virus and parainfluenza virus 3 in dual-purpose farms in Colima, Mexico

  • Daniel Figueroa-Chávez
  • José C. Segura-CorreaEmail author
  • Luís Jorge García-Márquez
  • Alfonso Pescador-Rubio
  • Arturo Gerardo Valdivia-Flores
Original Research


A cross-sectional study was carried out, from November 2007 to March 2008, to estimate the prevalence of and to determine risk factors associated with bovine syncytial respiratory virus (BRSV) and parainfluenza 3 virus (PIV3) in dual-purpose herds in Colima, México. One hundred and seventy-six sera from 33 herds for PIV3 and 232 sera from 44 herds for BRSV were used. Sera were analyzed by indirect ELISA for the detection of antibodies against BRSV and PIV3 in cattle herds to determine the seroprevalence of respiratory diseases. The apparent and true prevalences for PIV3 were 60.8% and 54.4% and for BRSV 52.2% and 50.8%, respectively. The percentage of herds showing at least one positive animal was 78.7% for PIV3, and 93.2% for BRSV. Age (≤12, 13–48, and >48 months old) and respiratory signs (no, yes) showed significant association (P < 0.05) with PIV3 and age with BRSV. This study showed that animals were exposed to both viruses and that age was the main risk factor. The need to establish new vaccination plans to effectively protect cattle against those infections in the state of Colima, Mexico is suggested.


Bovine respiratory disease complex Seroprevalence Risk factors Tropics 



The author would like to thank the reviewer comments to improve the manuscript.


  1. Antonis, A.F.G., de Jong, M.C., van der Poel, H.M., van der Most, R.G., Sotockhofe-Zurwieden, N., Kimman, T. and Schrijver, S., 2010. Age-dependent differences in the pathogenesis of bovine respiratory syncytial virus infections related to the development of natural immunocompetence. Journal of General Virology, 91, 2497-2506.PubMedCrossRefGoogle Scholar
  2. Barajas, R., 1995. Seroepidemiología de enfermedades infecciosas en ganado bovino en el trópico mexicano. En: Memorias del XIX Congreso Nacional de Buiatría, Coahuila, México. pp. 9-27.Google Scholar
  3. Costa, M., García, L., Yunus, A., Rockemann, D. Samal, S. and Cristina, J., 2000. Bovine respiratory syncytial virus: first serological evidence in Uruguay. Veterinary Research, 31, 241-246.PubMedCrossRefGoogle Scholar
  4. Ellis, J., 2001. The immunology of bovine respiratory disease complex. Veterinary Clinics of North American: Food Animal Practice, 17, 535-550.Google Scholar
  5. Graham, D.A., McShane, J., Mawhinney, K.A., McLaren, L.E., Adair, B.M. and Merza, M., 1998. Evaluation of a single ELISA system for detection of seroconversion to bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza-3 virus, and infectious bovine rhinotracheitis virus: comparison with testing by virus neutralization and hemagglutination inhibition. Journal of Veterinary Diagnostic Investigation, 10, 43-48.PubMedCrossRefGoogle Scholar
  6. Grubbs, T., Kania, A. and Potgieter, D., 2001. Prevalence of ovine and bovine respiratory syncytial virus infections in cattle determined with a synthetic peptide-based immunoassay. Journal of Veterinary Diagnostic Investigation, 13, 128-132.PubMedCrossRefGoogle Scholar
  7. Gulliksen, S.M., Jor, E., Lie, K.I., Løken, T., Akerstedt, J. and Østerås, O., 2009. Respiratory infections in Norwegian dairy calves. Journal of Dairy Science, 92, 5139-5146.PubMedCrossRefGoogle Scholar
  8. Hägglund, S., Svenson, C., Emanuelson, U., Valarcher, J. and Alenius, S., 2006. Dynamics of virus infection involved in the bovine respiratory disease complex in Swedish dairy herds. Veterinary Journal, 172, 320-328.CrossRefGoogle Scholar
  9. Härtel, H., Nikunen, S., Neuvonen, E., Tanskanen, R., Kivela, S., Aho, P., Soveri, T. and Saloniemi, H., 2004. Viral and bacterial pathogens in bovine respiratory disease in Finland. Acta Veterinaria Scandinavica, 45, 193-200.PubMedCrossRefGoogle Scholar
  10. INEGI. 2006. Anuario Estadístico del estado de Colima. (Instituto Nacional de Estadística, Geografía e Informática. Aguascalientes México).Google Scholar
  11. Juárez, F., Trigo, F., Chávez, G. and Vargas, R., 2003. Identificación de agentes virales por inmunohistoquímica en enfermedades respiratorias de bovinos en corral de engorda. Veterinaria México, 34, 1-12.Google Scholar
  12. Magaña, U., Solorio, J. and Segura, J.C., 2005. Rinotraqueitis infecciosa bovina en hatos lecheros de la región de Cotzio-Téjaro, Michoacán, México. Técnica Pecuaria en México, 43, 27-37.Google Scholar
  13. Maillard, R.; Assié, S. and Douart, A., 2006. Respiratory disease in adult cattle. Unité pathologie du Bétail, Ecole Nationale Vétérinaire, Alfort France.Google Scholar
  14. Obando, C., Hidalgo, M., Merza, M., Montoya, A., Klingeborn, J. and Moreno L., 1999. Seroprevalence to bovine virus diarrhoea virus and other viruses of the bovine respiratory complex in Venezuela (Apure State). Preventive Veterinary Medicine, 41, 271-278.PubMedCrossRefGoogle Scholar
  15. Odeón, A., Spath, E., Paloma, E., Leunda, M., Sainz, F., Pérez, S., Kaiser, G., Draghi, M., Cetra, B. and Cano, A., 2001. Seroprevalencia de la diarrea viral bovina, herpes virus bovino y virus syncytial respiratorio en Argentina. Revista de Medicina Veterinaria, 82, 216-220.Google Scholar
  16. Ohlson, A., Heuer, C., Lockhart, C., Tråvén, M., Emanuelson, U. and Alenius, S., 2010. Risk factors for seropositivity to bovine corona virus and bovine respiratory syncytial virus in dairy herds. Veterinary Record, 167, 201-207.PubMedCrossRefGoogle Scholar
  17. Richey, E., 2002. Bovine respiratory syncytial virus (VRSB) and parainfluenza-3 (PI-3). University of Florida, IFAS, Extensión, pp. 1-7.Google Scholar
  18. SAS., 2007. SAS User’s Guide. Statistics. SAS Institute Inc. Cary, NC, USA.Google Scholar
  19. Shirvani, E., Lotfi, M., Kamalzadeh, M., Noaman, V., Bahriari, M., Morovati, B. and Hatami, A., 2011. Seroepidemiological study of bovine respiratory viruses (BRSV, BoHV-1, PI-3V, BVDV, and BAV-3) in dairy cattle in central región of Iran (Esfahan province). Tropical Animal Health and Production, doi: 10.1007/s11250-011-9908-z
  20. Snowder, G., Van Vleck, L., Cundiff, L. and Bennett, G., 2006. Bovine respiratory disease in feedlots cattle: Environmental, genetic, and economic factors. Journal of Animal Science, 84, 1999-2008.PubMedCrossRefGoogle Scholar
  21. Solís-Calderón, J.J., Segura-Correa, J.C., Aguilar-Romero, F. and Segura-Correa, V.M., 2007. Detection of antibodies and risk factors for infection with bovine respiratory syncytial virus and parainfluenza virus-3 in beef cattle of Yucatan, Mexico. Preventive Veterinary Medicine, 82, 102-110.PubMedCrossRefGoogle Scholar
  22. Thrusfield, M., 2007. Veterinary epidemiology. 3rd ed. (Blackwell Publishing Ames, Iowa).Google Scholar
  23. Trigo, F., 1987. El complejo respiratorio infeccioso de los bovinos y ovinos. Ciencia Veterinaria, 4, 1-36.Google Scholar
  24. Valarcher, J.F. and Taylor, G., 2007. Bovine respiratory syncytial virus infection. Veterinary Research, 38, 153-180.PubMedCrossRefGoogle Scholar
  25. Yavru, S., Simsek, A., Yapkic, O. and Kale, M., 2005. Serological evaluation of viral infections in bovine respiratory tract. Acta Veterinaria Beograd, 55, 219-226.CrossRefGoogle Scholar
  26. Yesilbag, K. and Guengoer, B., 2008. Seroprevalence of bovine respiratory viruses in North-Western Turkey. Tropical Animal Health and Production, 40, 55-60.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Daniel Figueroa-Chávez
    • 1
  • José C. Segura-Correa
    • 2
    Email author
  • Luís Jorge García-Márquez
    • 1
  • Alfonso Pescador-Rubio
    • 1
  • Arturo Gerardo Valdivia-Flores
    • 1
  1. 1.Centro Universitario de Investigación y Desarrollo Agropecuario de la Universidad de ColimaColimaMexico
  2. 2.Facultad de Medicina Veterinaria y Zootecnia de la Universidad Autónoma de YucatánMéridaMexico

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