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Determination of bovine rotavirus G serotype by polymerase chain reaction

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Abstract

A total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by RNA-PAGE for the detection of presence of rotavirus. RNA-PAGE analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. Out of 68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. Similarly, out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. Rotavirus-positive samples represented long electropherotype. All RNA-PAGE-positive faecal samples for rotavirus subjected to RT-PCR for VP7 gene, ten samples yielded a specific product of 1,013 bp of VP7 gene. All the PCR-positive samples of the present study were subjected to genotyping with primers for G6, G8 and G10 genotype. All positive samples showed G10 genotype. This indicates that G10 may be predominant genotype among bovine calves in Mumbai region in India.

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Acknowledgements

The authors thank the associate dean of Bombay Veterinary College, Parel, Mumbai; Dr. Soumendu Chakravarti, scientist, Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus; and Dr. Ranjit Kumar Aich, research associate, Department of Medicine, WBUAFS, Kolkata.

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Authors and Affiliations

  1. Department of Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, 700037, India

    Anjan Mondal

  2. West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, 700037, India

    Ranjit Aich

  3. Bombay Veterinary College, Parel, Mumbai, 400012, India

    Sharmila Majee & Anilkumar Sadashivrao Bannalikar

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  1. Anjan Mondal
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  2. Ranjit Aich
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  3. Sharmila Majee
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  4. Anilkumar Sadashivrao Bannalikar
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Correspondence to Anjan Mondal.

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Mondal, A., Aich, R., Majee, S. et al. Determination of bovine rotavirus G serotype by polymerase chain reaction. Trop Anim Health Prod 44, 763–767 (2012). https://doi.org/10.1007/s11250-011-9962-6

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