Skip to main content
Log in

Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey

  • Brief Communication
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

All pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep and pigs. Besides the important economical losses, pestiviruses may compromise the normal immune response to other pathogens and increase the severity of other infections in sheep. In this study, aborted foetuses (cattle and sheep) in either coastal or inland Black Sea region of Turkey were surveyed for the presence of RNA from pestiviruses (bovine viral diarrhoea virus (BVDV), border disease virus (BDV)). The presence of BVDV RNA was found in 6 of 21 aborted calves (28.57%), although BDV RNA was detected in 14 of 21 aborted lambs (66.66%) by reverse transcriptase polymerase chain reaction. This study also investigates the distribution of viral RNA within the brain, liver and lung of aborted foetuses. The viral RNA positivity rates for the organs varied and were as follows: brain 40.47% and liver and lung 38.09%. The results revealed that pestiviruses are important abort pathogen in the provinces of northern Turkey.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Abbreviations

BDV:

Border disease virus

BVDV:

Bovine viral diarrhoea virus

RT-PCR:

Reverse transcriptase polymerase chain reaction

References

  • Carpenter, T.E., Chrıèl, M., Andersen, M.M., Wulfson, L., Jensen, A.M., Houe, H. and Greiner, M., 2006. An epidemiologic study of late-term abortions in dairy cattle in Denmark, July 2000–August 2003, Preventive Veterinary Medicine 77, 215–229

    Article  PubMed  Google Scholar 

  • De Mia, G.M., Greiser-Wilke, I., Feliziani, F., Giammarioli, M. and De Giuseppe, A., 2005. Genetic characterization of a caprine pestivirus as the first member of a putative novel pestivirus subgroup, Journal of Veterinary Medicine B—Infectious Diseases and Veterinary Public Health, 52, 206–210

    Article  Google Scholar 

  • Dubois, E., Russo, P., Prigent, M. and Thiery, R., 2008. Genetic characterization of ovine pestiviruses isolated in France, between 1985 and 2006, Veterinary Microbiology, 130, 69–79

    Article  PubMed  CAS  Google Scholar 

  • Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U. and Ball, L.A., 2005. Family Flaviviridae. In: C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger and L.A. Ball (eds), Virus taxonomy—classification and nomenclature of viruses. Eight report of the International Committee on the Taxonomy of Viruses. Elsevier Academic, San Diego

    Google Scholar 

  • Finlaison, D.S., King, K.R., Frost, M.J. and Kirkland P.D., 2009. Field and laboratory evidence that Bungowannah virus, a recently recognised pestivirus, is the causative agent of the porcine myocarditis syndrome (PMC), Veterinary Microbiology, 136, 259–265

    Article  PubMed  Google Scholar 

  • Greiser-Wilke, I., Haas, L., Dittmar, K., Liess, B. and Moennig, V., 1993. RNA insertions and gene duplications in the nonstructural protein p125 region of pestivirus strains and isolates in vitro and in vivo, Virology, 193, 977–980

    Article  PubMed  CAS  Google Scholar 

  • Gur, S., 2009. A investigation of border disease virus in sheep in western Turkey, Tropical Animal Health Production, 41, 1409–1412

    Article  CAS  Google Scholar 

  • Hasircioglu, S., Kale, M. and Acar, A., 2009. Investigation of pestiviruses infections in aborted sheep and goats in Burdur region, Kafkas Universitesi Veteriner Fakultesi Dergisi, 15, 163–167

    Google Scholar 

  • Lamm, C.G., Broaddus, C.C. and Holyoak, G.R., 2009. Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry, Veterinary Pathology, 46, 54–58

    Article  PubMed  CAS  Google Scholar 

  • Liu, L., Xia, H., Wahlberg, N., Belak, S. and Baule, C., 2009. Phylogeny, classification and evolutionary insights into pestiviruses, Virology, 385, 351–357

    Article  PubMed  CAS  Google Scholar 

  • Nagai, M., Hayashi M., Itou M., Fukutomi T., Akashi H., Kida H. and Sakoda Y. 2008. Identification of new genetic subtypes of bovine viral diarrhea virus genotype 1 isolates in Japan, Virus Genes, 36, 135–139

    Article  PubMed  CAS  Google Scholar 

  • Oguzoglu, C.T., Tan, M.T., Toplu, N., Demir, A.B., Dagalp, S.B., Karaoglu, T., Ozkul, A., Alkan, F., Burgu, I., Haas, L. and Greiser-Wilke, I., 2009. Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup?, Veterinary Microbiology, 135, 374–379

    Article  PubMed  CAS  Google Scholar 

  • Oguzoglu, C.T., Muz, D., Yilmaz, V., Alkan, F., Akca, Y. and Burgu, I., 2010. Molecular characterization of bovine virus diarrhea viruses species 2 (BVDV-2) from cattle in Turkey, Tropical Animal Health Production, 42, 1175–1180

    Article  Google Scholar 

  • Peterhans, E. and Schweizer, M., 2010. Pestiviruses: how to outmaneuver your hosts, Veterinary Microbiology, 142, 18–25

    Article  PubMed  Google Scholar 

  • Peterhans, E., Bachofen, C., Stalder, H. and Schweizer, M., 2010. Cytopathic bovine viral diarrhea viruses (BVDV): emerging pestiviruses doomed to extinction, Veterinary Research, 41, 1–10

    Article  Google Scholar 

  • Swasdipan, S., McGowan, M., Phillips, N. and Bielefeldt-Ohmann, H., 2002. Pathogenesis of transplacental virus infection: pestivirus replication in the placenta and foetus following respiratory infection, Microbial Pathogenesis, 32, 49–60

    Article  PubMed  CAS  Google Scholar 

  • Tan, M.T., Karaoglu, M.T., Erol, N. and Yildirim, Y., 2006. Serological and virological investigations of bovine viral diarrhoea virus (BVDV) infection in dairy cattle herds in Aydin province, Turkish Journal Veterinary and Animal Science, 30, 299–304

    Google Scholar 

  • Thabti, F., Fronzaroli, L., Dlissi, E., Guibert, J.M., Hammami, S., Pepin, M. and Russo, P., 2002. Experimental model of border disease virus infection in lambs: comparative pathogenicity of pestiviruses isolated in France and Tunisia, Veterinary Research, 33, 35–45

    Article  PubMed  Google Scholar 

  • Thabti, F., Letellier, C., Hammami, S., Pepin, M., Ribiere, M., Mesplede, A., Kerkhofs, P. and Russo, P., 2005. Detection of a novel border disease virus subgroup in Tunisian sheep, Archives of Virology, 150, 215–229

    Article  PubMed  CAS  Google Scholar 

  • Vilcek, S. and Paton, D.J., 2000. A RT-PCR assay for the rapid recognition of border disease virus, Veterinary Research, 31, 437–445

    Article  CAS  Google Scholar 

  • Yesilbag, K., Förster, C., Bank-Wolf, B., Yilmaz, Z., Alkan, F., Ozkul, A., Burgu, I., Rosales, S.C., Thiel, H.J. and Konig, M., 2008. Genetic heterogeneity of bovine viral diarrhoea virus (BVDV) isolates from Turkey: identification of a new subgroup in BVDV-1, Veterinary Microbiology, 130, 258–267

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Harun Albayrak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Albayrak, H., Gumusova, S.O., Ozan, E. et al. Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey. Trop Anim Health Prod 44, 677–680 (2012). https://doi.org/10.1007/s11250-011-9955-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-011-9955-5

Keywords

Navigation