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Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection

Abstract

Recently, we found that Holstein cattle (Bos taurus taurus) displayed higher skin test prevalence and disease severity compared to zebu (Bos taurus indicus) herd-mates kept under identical husbandry conditions in Ethiopia. To determine whether these susceptibility differences were patent at the level of the innate immune system, we infected monocytes from naïve Holstein or Sahiwal zebu cattle with either live virulent Mycobacterium tuberculosis or Mycobacterium bovis. The cytokine profile following infection was compared between the two breeds by measuring IL-1α, IL-6, IL-10, IL-12 and TNF-α as well as nitric oxide in culture supernatants. Our results suggested subtle differences in the cytokine profile because only infection-induced IL-6 production was significantly increased in monocytes from the more susceptible Holstein cattle.

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Acknowledgements

We would like to thank Dr C. Garcia-Pelayo (VLA Weybridge) for providing mycobacterial stocks for the infection experiments. The work was supported by the Wellcome Trust. We also like to thank Dr Jayne Hope (Institute for Animal Health, Compton, UK) for the supply of bovine cytokines. Dr. Glass received financial support from the Biotechnology and Biological Sciences Research Council (BBSRC) through Institute Strategic Grant funding as well as Grant number 215/S15354, and the Wellcome Trust ‘Animal Health in the Developing World Initiative’ grant. [Grant number 075820/A/04/Z].

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Vordermeier, M., Ameni, G. & Glass, E.J. Cytokine responses of Holstein and Sahiwal zebu derived monocytes after mycobacterial infection. Trop Anim Health Prod 44, 651–655 (2012). https://doi.org/10.1007/s11250-011-9950-x

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  • DOI: https://doi.org/10.1007/s11250-011-9950-x

Keywords

  • Cattle
  • Zebu
  • Holstein
  • Mycobacterium tuberculosis
  • Mycobacterium bovis
  • Cytokines
  • Bovine tuberculosis