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Estimation of the diagnostic accuracy of the glyco-C and US9 gene-based polymerase chain reaction technique for the detection of bovine Herpesvirus type 5 DNA in decomposed brain suspension from a slaughter house using Bayesian analysis, Brazil

Tropical Animal Health and Production volume 44, pages 379–382 (2012)Cite this article

Abstract

Brazil represents the greatest beef producer among tropical countries, and the major obstacle for meat international trade is sanitary problems especially closely related to viral encephalitis. The goal of this study was to estimate the accuracy of the glycol and US9 gene-based polymerase chain reactions (PCRs) for the detection of bovine Herpesvirus type 5 (BoHV-5) from decomposed brain samples (n = 95). For this purpose, a latent-class (Bayesian) approach was used. Sensitivity (Se) was estimated to be 70% (95% probability interval, 40–80) and specificity (Sp) 100% in the statistical analysis for both PCRs used. Accordingly, a minimum of ≥40% of the calves was estimated to harbor BoHV-5 DNA even after 72 h of decomposition at room temperature. It was concluded that US9 gene-based PCR could also be considered a cost-effective alternative in sanitary programmers. However, given the importance of veterinary diagnoses, PCR-positive samples should be further confirmed through in vitro isolation and/or sequencing.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil. TC Cardoso is a fellowship of CNPq.

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Authors and Affiliations

  1. Laboratório de Viroses Bovídeos, Instituto Biológico, São Paulo, São Paulo, Brazil

    Adriana Hellmeister de Campos Nogueira

  2. Laboratory of Animal Virology, Faculty of Veterinary Medicine, Rua Clóvis Pestana, 793, 16.050-680, Araçatuba, São Paulo, Brazil

    Tereza Cristina Cardoso, Talita Fontes Antello, Flávia Volpato Vieira & Saulo Tarso Zacarias Machado

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  1. Tereza Cristina Cardoso
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  2. Talita Fontes Antello
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  3. Flávia Volpato Vieira
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  4. Saulo Tarso Zacarias Machado
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  5. Adriana Hellmeister de Campos Nogueira
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Correspondence to Tereza Cristina Cardoso.

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Cardoso, T.C., Antello, T.F., Vieira, F.V. et al. Estimation of the diagnostic accuracy of the glyco-C and US9 gene-based polymerase chain reaction technique for the detection of bovine Herpesvirus type 5 DNA in decomposed brain suspension from a slaughter house using Bayesian analysis, Brazil. Trop Anim Health Prod 44, 379–382 (2012). https://doi.org/10.1007/s11250-011-9929-7

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  • DOI: https://doi.org/10.1007/s11250-011-9929-7

Keywords

  • Bovine Herpesvirus type 5
  • Gold standard
  • Diagnosis