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Molecular typing of Brucella species isolates from livestock and human

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Abstract

Although host specificity has been observed in different species of Brucella, crossing the animal host boundary is likely to occur at any time. In this study, Bruce ladder PCR and abortus–melitensis–ovis–suis (AMOS) PCR assays were used to characterize 47 Brucella isolates from Indian origin in order to know exact species for understanding epidemiology of brucellosis. Out of them, 28, 14, and 5 isolates were found to be Brucella abortus, Brucella melitensis, and Brucella suis, respectively. Further analysis by AMOS PCR has identified that all the B. abortus isolates belong to any one of the biovar 1, 2, or 4; of the five B. suis isolates, three belong to biovar 1 and two belong to any one of the biovar 2, 3, 4, or 5. Although this multiplex Bruce ladder PCR is useful in differentiating all Brucella species, elaborate study is required to further characterize the isolates at exact biovar level.

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Acknowledgments

We are thankful to the Deputy Director General (Animal Sciences) and Assistant Director General (Animal Health), ICAR, New Delhi for providing the facilities, support, and encouragement.

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Authors and Affiliations

  1. Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Hebbal, Bangalore, 560 024, India

    Mohandoss Nagalingam, Rajeswari Shome, Vinayagamurthy Balamurugan, Bibek Ranjan Shome, Krishnamsetty NarayanaRao & Krishnamsetty Prabhudas

  2. Department of Microbiology, Veterinary College, KAVFSU, Hebbal, Bangalore, 560 024, India

    Vivekananda & Shrikrishna Isloor

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  1. Mohandoss Nagalingam
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  2. Rajeswari Shome
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  3. Vinayagamurthy Balamurugan
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  4. Bibek Ranjan Shome
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  5. Krishnamsetty NarayanaRao
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  6. Vivekananda
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  7. Shrikrishna Isloor
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  8. Krishnamsetty Prabhudas
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Correspondence to Rajeswari Shome.

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Nagalingam, M., Shome, R., Balamurugan, V. et al. Molecular typing of Brucella species isolates from livestock and human. Trop Anim Health Prod 44, 5–9 (2012). https://doi.org/10.1007/s11250-011-9886-1

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  • DOI: https://doi.org/10.1007/s11250-011-9886-1

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