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Reproductive failure in wild boars associated to porcine parvovirus infection and in vivo and in vitro characterization of the causal isolate

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Abstract

Investigations were made to identify the causal agent of an acute outbreak of abortions in a domesticated herd of wild boar. Only porcine parvovirus (PPV) was isolated from samples of organs from the still-born sucklings and mummified aborted fetuses. The isolated virus hemagglutinated erythrocytes of guinea pig, murine, rat, and chicken. Identity of the virus, designated the BQ strain, was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results from ELISA, PCR, immunofluorescence assay, and electron microscopy. PPV BQ strain was adapted to growth in a swine testicular cell line. When inoculated into healthy sows, PPV BQ caused the same reproductive disorder observed in the affected herd.

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Acknowledgments

The study was partly supported by funds from the Chinese State Key Laboratory of Veterinary Biotechnology Fund (NKLVBP200807).

Conflict of interest statement

None of the authors of this paper has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.

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Authors and Affiliations

  1. Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, 150001, China

    Chao-fan Zhang, Chang-mu Chen, Shang-jin Cui & Lan-fei Miao

  2. Laboratory of Marine Genetics and Breeding (MGB), Ocean University of China, Qingdao, 266003, China

    Cui-Ping Song

  3. China Animal Health and Epidemiology Center, Qingdao, 266032, China

    Cui-Ping Song

Authors
  1. Chao-fan Zhang
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  2. Cui-Ping Song
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  3. Chang-mu Chen
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  4. Shang-jin Cui
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  5. Lan-fei Miao
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Corresponding author

Correspondence to Shang-jin Cui.

Additional information

Chaofan Zhang and Cuiping Song contributed equally to the authorship of this work.

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Zhang, Cf., Song, CP., Chen, Cm. et al. Reproductive failure in wild boars associated to porcine parvovirus infection and in vivo and in vitro characterization of the causal isolate. Trop Anim Health Prod 42, 1611–1613 (2010). https://doi.org/10.1007/s11250-010-9644-9

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  • DOI: https://doi.org/10.1007/s11250-010-9644-9

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