Abstract
Breeding records, including 649 inseminations during fall and winter at a dairy farm in a subtropical area of Western Mexico (24º N; 24°C, mean annual temperature 24°C) were analyzed to document effects of sex-sorted semen from commercial Gyr bullls, estrus synchronization protocol, inseminator, sire and environmental conditions on fertility of crossbred cows (Holstein × Gyr). Percentage of services resulting in pregnancies decreased sharply when sex-sorted semen was used (22.7 vs. 37.7%; P < 0.01). Although statistically not significant (P = 0.31), cows whose first insemination was in November experienced a numerically greater reduction (21 percentage points) in pregnancy rate compared to cows whose first insemination occurred in December. Substantial increases in services per pregnancy (4.71 ± 1.35 vs. 2.13–2.43; P < 0.01) were associated with the warmer month of the study period, November, compared to other winter months. Pregnancy rates of cows regardless of semen category (33%) were not affected by sire, temperature–humidity index and estrus synchronization protocol. Cows inseminated by one inseminator had higher pregnancy rates (P < 0.01) than cows inseminated by other two technicians. The sorted sperm produced 91% (142/156) female offspring. It was concluded that, under the field conditions of the present study, pregnancy rate with sexed semen was 15 percentage points lower than pregnancy rates using conventional semen, with 91% of female calves derived from sexed sperm.
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Andersson, M., Taponen, J., Kommeri, M., Dahlbom, M., 2006. Pregnancy rates in lactating Holstein–Friesian cows after artificial insemination with sexed sperm. Reproduction in Domestic Animals, 41, 95–97.
Avendaño-Reyes L., Fuquay, J.W., Moore, R.B., Liu, Z., Clark, B.L., Vierhout, C., 2010. Relationship between accumulated heat stress during the dry period, body condition score, and reproduction parameters of Holstein cows in tropical conditions. Tropical Animal Health and Production, 42, 265–273.
Badinga, L., Collier, R.J., Thatcher, W.W., Wilcox, C.J., 1985. Effects of climatic and management factors on conception rate of dairy cattle in subtropical environment. Journal of Dairy Science, 68, 78–85.
Cerchiaro, I., Cassandro, M., Dal Zotto, R., Carnier, P., Gallo, L., 2007. A field study on fertility and purity of sex-sorted cattle sperm. Journal of Dairy Science, 90, 2538–2542.
De Graaf, S.P., Evans, G., Maxwell, W.M.C., O’Brien, J.K., 2006. In vitro function of fresh and frozen-thawed ram spermatozoa after sex sorting and re-freezing. Reproduction Fertility and Development, 18, 867–874.
De Graaf, S.P., Leahy, T., Marti J.I., Evans G., Maxwell W.M.C., 2008. Application of seminal plasma in sex-sorting and sperm cryopreservation. Theriogenology, 70, 1360–1363.
DeJarnette, J.M., Nebel, R.L., Marshall, C.E., Moreno, J.F., McCleary, C.R., Lenz, R.W., 2008. Effect of sex-sorted sperm dosage on conception rates in Holstein heifers and lactating cows. Journal of Dairy Science, 91, 1778–1785.
De Vries, A., Steenholdt, C., Risco, C.A., 2005. Pregnancy rate and milk production in natural service and artificially inseminated dairy herds in Florida and Georgia. Journal of Dairy Science, 88, 948–956.
De Vries, A., Overton, M., Fetrow, J., Leslie, K., Eicker, S., Rogers, G., 2008. Exploring the impact of sexed semen on the structure of the dairy industry. Journal of Dairy Science, 91, 847–856.
Frijters, A.C.J., Mullaart, E., Roelofs, R.M.G., van Hoorne, R.P., Moreno, J.F., Moreno, O., Merton, J.S., 2009. What affects fertility more, low sperm dosage or the sorting process? Theriogenology, 71, 64–67.
Garner, D.L., Seidel Jr, G.E., 2008. History of commercializing sexed semen for cattle. Theriogenology, 69, 886–895.
Gaughan, J.B., Mader, T.L., Holt, S.M., Sullivan, M.L., Hahn, G.L., 2009. Assessing the heat tolerance of 17 beef cattle genotypes. International Journal of Biometeorology, on line ahead of print: http://www.springerlink.com/content/j3hgp51qg7271q2v/
Huang, C., Tsuruta, S., Bertrand, J.K., Misztal, I., Lawlor, T.J., Clay, J.S., 2008. Environmental effects on conception rates of Holsteins in New York and Georgia. Journal of Dairy Science, 91, 818–825.
McDowell, D., Hooven, N., Cameron, K., 1979. Effects of climate on performance of Holsteins in first lactation. Journal of Dairy Science, 68, 2418–2435.
McManus, C., Prescott, E., Paludo, G.R., Bianchini, E., Louvandini, H., Mariante, A.S., 2009. Heat tolerance in naturalized Brazilian cattle breeds. Livestock Science, 120, 256–264.
National Research Council, 2001. Nutrient Requirements of Dairy Cattle. 7th revised edition. National Academy of Science, Washington, DC.
Rath, D., Johnson, L.A., 2008. Application and commercialization of flow cytometrically sex-sorted semen. Reproduction in Domestic Animals, 43, 338–346.
Roy, J.H.B., 2007. Rearing dairy herd replacements. International Journal of Dairy Technology, 31, 73–79.
Schenk, J.L., Everett, R.W., 2007. Insemination of Holstein cows with sexed sperm. Journal of Dairy Science, 90(Suppl. 1):18. (Abstract).
Seidel Jr., G.E., Garner, D.L., 2002. Current status of sexing mammalian spermatozoa. Reproduction, 124, 733–743.
Seidel G.E., Schenk, J.L., 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: effects of sperm numbers per inseminate and site of sperm deposition. Animal Reproduction Science, 105, 129–38.
Seidel Jr, G., Schenk, J., Herickhoff, L., Doyle, S., Brink, Z., Green, R., Cran, D., 1999. Insemination of heifers with sexed sperm. Theriogenology, 52, 1407–1420.
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Mellado, M., Coronel, F., Estrada, A. et al. Fertility in Holstein × Gyr cows in a subtropical environment after insemination with Gyr sex-sorted semen. Trop Anim Health Prod 42, 1493–1496 (2010). https://doi.org/10.1007/s11250-010-9585-3
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DOI: https://doi.org/10.1007/s11250-010-9585-3