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Phenotypic and genotypic properties of Escherichia coli isolated from colisepticemic cases of Japanese quail

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Abstract

This study was conducted to characterize the Escherichia coli isolates from colisepticemic Japanese quails. One hundred and nine E. coli were isolated in pure culture from heart blood of dead Japanese quails. The sampled birds were originated from four different farms. Antibiotic resistance pattern of E. coli isolates were determined against nine antibacterial agents. Phylotype and virulence genes of the isolates were detected by polymerase chain reaction. By disc diffusion method, all of the isolates showed resistance to three or more antibiotics, and 19 different patterns of multiple drug resistance were observed. Phylotyping of the most prevalent multiple drug-resistant isolates revealed that they mostly belonged to phylogroups A (A1 subgroup). The E. coli isolates belong to four phylogenetic groups: A (55.0%), B1 (18.3%), B2 (17.4%), and D (9.2%). Eighty-nine (81.7%) isolates were distributed in five phylogenetic subgroups including 22 (20.2%) in A0, 38 (34.9%) in A1, 19 (17.4%) in B23, 7 (6.4%) in D1, and 3 (2.8%) in D2. The examined E. coli isolates exhibit at least one of the virulence genes tested, whereas three most prevalent genes were crl (94.5%), fimH (89.0%), and iutA (51.4%), respectively. The genetic marker for Afa (afaI B-C), S (sfa/focD-E), and P (papE-F) fimbriae were found in one, four, and ten isolates, respectively. Thirteen different combinations of virulence gene were observed, where combination of crl and fimH genes was the most prevalent pattern. None of the isolates contained the ipaH, stx1, stx2, and eaeA genetic markers. In conclusion, E. coli strains could be considered as a causative agent of mortality in quail farms. In conclusion, E. coli isolates from colisepticemic quails are distributed in different phylogroups, are resistant to combinations of antibiotic agents, and contain several virulence genes.

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Acknowledgments

This work was supported by a grant from Shahid Bahonar University of Kerman.

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Authors and Affiliations

  1. Department of Poultry Diseases, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O. Box 7616914111, Kerman, Iran

    Mahmood Salehi

  2. Department of Microbiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    Reza Ghanbarpour

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  1. Mahmood Salehi
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  2. Reza Ghanbarpour
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Correspondence to Mahmood Salehi.

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Salehi, M., Ghanbarpour, R. Phenotypic and genotypic properties of Escherichia coli isolated from colisepticemic cases of Japanese quail. Trop Anim Health Prod 42, 1497–1504 (2010). https://doi.org/10.1007/s11250-010-9583-5

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