Abstract
Anaplasma species are transmitted by ticks and cause diseases in humans and animals. These pathogens infect sheep, an economically important domestic animal worldwide. The current study was designed to characterize in 200 animals the infection with Anaplasma phagocytophilum and Anaplasma ovis and the genetic diversity of A. ovis strains collected from a naturally infected sheep flock with poor health condition. Sheep had 98% seroprevalence to Anaplasma spp. antibodies. PCR results confirmed the presence of A. phagocytophilum and A. ovis DNA in 11.5% and 37% of the sheep, respectively. Concurrent infections were detected in 6.5% of the sheep. Seventy-one adult ticks were collected from 45 sheep with infestations ranging from one to 15 ticks per animal. The analysis of A. ovis msp4 sequences demonstrated a previously unreported polymorphism for this pathogen with 17 different haplotypes in infected sheep. These results demonstrated that, although A. ovis msp4 haplotypes may be less variable when compared with Anaplasma marginale and A. phagocytophilum strains on a global scale, genetic polymorphisms occur in this locus in strains obtained from an infected sheep flock with poor health condition.
References
Caracappa, S. 1999. Livestock production and animal health in Sicily, Italy. Parassitologia 41, 17–23.
de la Fuente, J., Naranjo, V., Ruiz-Fons, F., Höfle, U., Fernández de Mera, I.G., Villanúa, D., Almazán, C., Torina, A., Caracappa, S., Kocan, K.M., Gortázar, C. 2005a. Potential vertebrate reservoir hosts and invertebrate vectors of Anaplasma marginale and A. phagocytophilum in central Spain. Vector Borne Zoonotic Dis. 5, 390–401.
de la Fuente, J., Torina, A., Naranjo, V., Caracappa, S., Di Marco, V., Alongi, A., Russo, M., Maggio, A.R., Kocan, K.M. 2005b. Infection with Anaplasma phagocytophilum in a seronegative patient in Sicily, Italy: Case Report. Ann. Clin. Microbiol. Antimic. 4, 15.
de la Fuente, J., M.W. Atkinson, V. Naranjo, I.G. Fernández de Mera, A.J. Mangold, K.A. Keating, and K.M. Kocan, 2007. Sequence analysis of the msp4 gene of Anaplasma ovis strains. Vet. Microbiol. 119, 375–381.
Dumler, J.S., Barbet, A.C., Bekker, C.P.J., Dasch, G.A., Palmer, G.H., Ray, S.C., Rikihisa, Y., Rurangirwa, F.R. 2001. Reorganization of the genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions subjective synonyms of Ehrlichia phagocytophila. Int. J. Sys. Evol. Microbiol. 51, 2145–2165.
Friedhoff, K.T. 1997. Tick-borne diseases of sheep and goats caused by Babesia, Theileria or Anaplasma spp. Parassitologia 39, 99–109.
Knowles, D.P., Torioni de Echaide, S., Palmer, G.H., McGuire, T., Stiller, D., McElwain, T.F. 1996. Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle. J. Clin. Microbiol. 34, 2225–2230.
Kocan, K.M., de la Fuente, J., Blouin, E.F., Garcia-Garcia, J.C. 2004. Anaplasma marginale (Rickettsiales: Anaplasmataceae): Recent advances in defining host–pathogen adaptations of a tick-borne Rickettsia. Parasitol. 129, S285–S300.
Manilla, G. 1998. Fauna D’Italia. Acari: Ixodida. Calderini, Bologna, Italy.
Ndung'u, L.W., Aguirre, C., Rurangirwa, F.R., McElwain, T.F., McGuire, T.C., Knowles, D.P., Palmer, G.H. 1995. Detection of Anaplasma ovis infection in goats by major surface protein 5 competitive inhibition enzyme-linked immunosorbent assay. J. Clin. Microbiol. 33, 675–679.
Scoles, G.A., Goff, W.L., Lysyk, T.J., Lewis, G.S., Knowles, D.P. 2008. Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates. Vet. Microbiol. 130, 184–190.
Splitter, E.J., Anthony, H.D., Twiehaus, M.J. 1956. Anaplasma ovis in the United States: experimental studies with sheep and goats. Am. J. Vet. Res. 17, 487–491.
Tibbitts, T., Goff, W.L., Foreyt, W., Stiller, D. 1992. Susceptibility of two rocky mountain bighorn sheep to experimental infection with Anaplasma ovis. J. Wildl. Dis. 28, 125–129.
Torina, A., Vicente, J., Alongi, A., Scimeca, S., Turlá, R., Nicosia, S., Di Marco, V., Caracappa, S., de la Fuente, J. 2007. Observed prevalence of tick-borne pathogens in domestic animals in Sicily, Italy during 2003–2005. Zoonoses Pub. Health 54, 8–15.
Torina, A., Alongi, A., Naranjo, V., Estrada-Peña, A., Vicente, J., Scimeca, S., Marino, A.M.F., Salina, F., Caracappa, S., de la Fuente, J. 2008a. Prevalence and genotypes of Anaplasma species and habitat suitability for ticks in a Mediterranean ecosystem. Appl. Env. Microbiol. 74, 7578–7584.
Torina, A., Alongi, A., Naranjo, V., Scimeca, S., Nicosia, S., Di Marco, V., Caracappa, S., Kocan, K.M., de la Fuente, J. 2008b. Characterization of Anaplasma infections in Sicily, Italy. Ann. N. Y. Acad. Sci. 1149, 90–93.
Torina, A., Alongi, A., Naranjo, V., Estrada-Peña, A., Vicente, J., Scimeca, S., Marino, A.M.F., Monteverde, V., Caracappa, S., de la Fuente, J. 2009. Prevalence of Anaplasma species and habitat suitability for ticks in Sicily. Clin. Microbiol. Infect. 15 (Suppl. 2), 57-58.
Acknowledgments
This study was supported by the Italian Ministry of Health (project RC IZS SI 2004) and Ministerio de Ciencia e Innovación (project BFU2008-01244/BMC), CSIC intramural project 200830I249 to JF, Ministerio de Educación y Ciencia (MEC; project AGL2005-07401), and FEDER, Spain. R.C. Galindo was funded by MEC, Spain.
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Torina, A., Galindo, R.C., Vicente, J. et al. Characterization of Anaplasma phagocytophilum and A. ovis infection in a naturally infected sheep flock with poor health condition. Trop Anim Health Prod 42, 1327–1331 (2010). https://doi.org/10.1007/s11250-010-9580-8
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DOI: https://doi.org/10.1007/s11250-010-9580-8