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Isolation and characterization of Indian isolates of camel pox virus

Abstract

In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains.

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Abbreviations

ATI:

A-type inclusion

CMLV:

Camel pox virus

CPE:

Cytopathic effect

MEGA 4:

Molecular evolutionary genetic analysis version 4

PCR:

Polymerase chain reaction

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Acknowledgement

The authors thank the Director, Indian Veterinary Research Institute, for providing the necessary facilities to carry out this work. This study was supported by grants from the Ministry of Forest and Environment (MOFE), Government of India, under the All India Coordinated Project on the Taxonomy Capacity Building of Poxviruses (AICOPTAX).

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Correspondence to Raj Kumar Singh.

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Bhanuprakash and Balamurugan equally contributed.

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Bhanuprakash, V., Balamurugan, V., Hosamani, M. et al. Isolation and characterization of Indian isolates of camel pox virus. Trop Anim Health Prod 42, 1271–1275 (2010). https://doi.org/10.1007/s11250-010-9560-z

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Keywords

  • Camel pox
  • Virus isolation
  • Identification
  • Characterization
  • PCR
  • Sequence analysis