In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains.
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Camel pox virus
- MEGA 4:
Molecular evolutionary genetic analysis version 4
Polymerase chain reaction
Al-Ziabi, O., Nishikawa, E. and Meyer, H., 2007. The first outbreak of camelpox in Syria. Journal of Veterinary Medical Science, 69, 541–543.
Balamurugan, V., Bhanuprakash, V., Hosamani, M., Srinivasan, V.A. and Singh, R. K., 2008. Comparative sequence analyses of B5R gene of Indian camelpox virus isolates with other orthopoxviruses, Indian Journal of Virology, 19, 34–38.
Balamurugan, V., Bhanuprakash, V., Hosamani, M., Kallesh, D.J., Venkatesan, G., Bina Chauhan and Singh, R. K., 2009. A polymerase chain reaction strategy for the diagnosis of camel pox, Journal of Veterinary Diagnostic Investigation, 21, 231–237.
Bera, B.C., Gnanavel, V., Barua, S., Shanmugasundaram, K., Gadvi, S., Yadav, V., Nagarajan, G., Bhanuprakash, V., Gulati, B. R., Kakker, N. K., Malik, P., Singh, R.V. , Sardarilal, Pathak K.M.L. and Singh R. K., 2010. Zoonotic camelpox virus infection in India, Abstract presented in the International Conference on Protecting Animal Health: Facilitating Trade in Livestock and Livestock Products, IAVMI, held at Raipur, Chhattisgarh, January 27-29, 197–198.
Chauhan, R..S. and Kaushik, R.K., 1987. Isolation of camelpoxvirus in India, British Veterinary Journal, 143, 581–582.
Hafez, S. M., Al-Sukayran, A., Dela Cruz, D., Mazloum, K. S., Al-Bokmy, A. M., Al-Mukayyel, A. and Amjad, A. M., 1992. Development of a live cell culture camelpox vaccine, Vaccine, 8, 533–539.
Kriz, B., 1982. A study of camelpox in Somalia, Journal of Comparative Pathology, 92, 1–8.
Leese, S., 1909. Two diseases of young camels, Journal of Tropical Veterinary Science, 4, 1.
Marodam V., Nagendrakumar, S.B., Tanwar, V.K., Thiagarajan, D., Reddy, G.S., Tanwar, R.K. and Srinivasan, V.A., 2006. Isolation and identification of camel pox virus, Indian Journal of Animal Science, 76, 326–327.
Meyer, H. and Rziha, H. J., 1993. Characterization of the gene encoding the A-type inclusion protein of camelpox virus and sequence comparison with other orthopoxviruses, Journal of General Virology, 74, 1679–1684.
Munz, E., Otterbein, C.K., Meyer, H. and Renner, I., 1997. A laboratory investigation to demonstrate a decreased virulence of two cell culture adapted African camelpox virus isolates as possible vaccine candidates, Journal of Camel Practice and Research, 4, 169–175.
Pfeffer, M., Meyer, H., Wernery, U. and Kaaden, O. R., 1996. Comparison of camelpox viruses isolated in Dubai, Veterinary Microbiology, 49, 135–146.
Ramyar, H. and Hessami, M., 1972. Isolation, cultivation and characterization of camel pox virus, Zentralbl Veterinarmed B, 19, 182–189.
Renner-Muller, I. C., Meyer, H. and Munz, E., 1995. Characterization of camelpoxvirus isolates from Africa and Asia, Veterinary Microbiology, 45, 371–381.
Tamura, K., Dudley, J., Nei, M. and Kumar, S., 2007. Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.
The authors thank the Director, Indian Veterinary Research Institute, for providing the necessary facilities to carry out this work. This study was supported by grants from the Ministry of Forest and Environment (MOFE), Government of India, under the All India Coordinated Project on the Taxonomy Capacity Building of Poxviruses (AICOPTAX).
Bhanuprakash and Balamurugan equally contributed.
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Bhanuprakash, V., Balamurugan, V., Hosamani, M. et al. Isolation and characterization of Indian isolates of camel pox virus. Trop Anim Health Prod 42, 1271–1275 (2010). https://doi.org/10.1007/s11250-010-9560-z
- Camel pox
- Virus isolation
- Sequence analysis