Skip to main content

Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India

Abstract

In this study, three outbreaks of peste des petits ruminants (PPR) in goats and sheep flocks with high morbidity and considerable mortality were recorded at Jhansi and Revati in Uttar Pradesh and Bhopal in Madhya Pradesh, India during 2003–2006. Clinical samples were collected from the affected flocks for laboratory investigation. The PPR virus (PPRV) antigen/nucleic acid in the infected tissues/swab materials was demonstrated by using sandwich enzyme-linked immunosorbent assay (ELISA) and reverse transcription–polymerase chain reaction techniques, and the antibody to PPRV in serum samples was detected by competitive ELISA. The causative agent of the outbreaks, PPRV, was successfully isolated in Vero cells at first passage itself, and its identity was confirmed. The isolated PPR viruses belong to lineage IV based on phylogenetic analysis of partial fusion gene sequences and are closely related to other Asian or Indian PPRV isolates/strains.

This is a preview of subscription content, access via your institution.

Fig. 1

Abbreviations

CPE:

Cytopathic effect

ELISA:

Enzyme-linked immunosorbent assay

MEGA 4:

Molecular evolutionary genetic analysis version 4

PBS:

Phosphate-buffered saline

PPRV:

peste des petits ruminants virus

DPI:

Days postinfection

RT–PCR:

Reverse transcription–polymerase chain reaction

References

  1. Abu-Elzein E. M. E., Housawi, F. M. T., Bashareek, Y., Gameel, A.A., Al-Afaleq, A.I. and Anderson, E., 2004. Severe PPR Infection in Gazelles Kept Under Semi-free Range Conditions. J Vet Med B Infect Dis Vet Public Health, 51, 68–71

    Google Scholar 

  2. Balamurugan, V., Sen, A., Saravanan, P., Singh, R.P., Singh, R.K., Rasool, T.J. and Bandhyopadhyay, S.K., 2006. One-step multiplex RT-PCR assay for the detection of PPR virus in clinical samples. Veterinary Research Communications, 30, 655–666

    Article  CAS  PubMed  Google Scholar 

  3. Couacy-Hymann, E., Roger, F., Hurard, C., Guillou, J.P., Libeau, G. and Diallo, A., 2002. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. Journal of Virological Methods, 100, 17–25

    Article  CAS  PubMed  Google Scholar 

  4. Dhar, P., Sreenivasa, B. P., Barrett, T., Corteyn, M., Singh, R. P. and Bandyopadhyay, S. K., 2002. Recent epidemiology of peste-des-petits-ruminants virus. Veterinary Microbiology, 88, 153–159

    Article  PubMed  Google Scholar 

  5. Forsyth, M. and Barrett, T., 1995. Detection and differentiation of rinderpest and peste des petits ruminants viruses in diagnostic and experimental samples by polymerase chain reaction using P and F gene-specific primers. Virus Research, 39, 151–163

    Article  CAS  PubMed  Google Scholar 

  6. George, A., Dhar, P., Sreenivasa, B.P., Singh, R.P. and Bandyopadhyay, S.K., 2006. The M and N genes-based simplex and multiplex PCRs are better than the F or H gene-based simplex PCR for Peste-des-petits-ruminants virus. Acta Virologica, 50, 217–222

    CAS  PubMed  Google Scholar 

  7. Shaila, M. S., Shamaki, D., Forsyth, M. A., Diallo, A., Groatley, L., Kitching, R. P. and Barrett, T., 1996. Geographic distribution and epidemiology of Peste-des-petits-ruminants viruses. Virus Research, 43, 149–153

    Article  CAS  PubMed  Google Scholar 

  8. Singh, R. P., Sreenivasa, B. P., Dhar, P, Shah, L. C. and Bandyopadhyay, S. K., 2004a. Development of monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants virus. Veterinary Microbiology, 98, 3–15

    Article  CAS  PubMed  Google Scholar 

  9. Singh, R. P., Sreenivasa, B. P., Dhar, P. and Bandyopadhyay. S. K., 2004b. A sandwich-ELISA for the diagnosis of Peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody. Archives of Virology, 149, 2155–2170

    Article  CAS  PubMed  Google Scholar 

  10. Singh, R.P., Saravanan, P., Sreenivasa, BP., Singh, R.K. and Bandyopadhyay, S.K., 2004c. Prevalence and distribution of peste des petits ruminants virus infection in small ruminants in India. Revue Scientifique et Technique, 23, 807–819

    CAS  PubMed  Google Scholar 

  11. Tamura, K., Dudley, J., Nei, M. and Kumar, S., 2007. Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank the Director of Indian Veterinary Research Institute for providing necessary facilities to carry out this work and the staff of National Morbillivirus Referral Laboratory, IVRI, Mukteswar for their valuable and timely help in carrying out this work. The study in the form of ad hoc scheme (F.No.11-3/2007-GA-II and 1-1/2007-ASR-IV) was funded by Indian Council of Agricultural Research, Government of India, New Delhi, India.

Author information

Affiliations

Authors

Corresponding author

Correspondence to V. Balamurugan.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Balamurugan, V., Sen, A., Venkatesan, G. et al. Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India. Trop Anim Health Prod 42, 1043–1046 (2010). https://doi.org/10.1007/s11250-010-9527-0

Download citation

Keywords

  • PPR
  • Outbreaks
  • Isolation
  • ELISA
  • RT–PCR assays
  • Phylogenetic analysis