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Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India

Tropical Animal Health and Production volume 42, pages 1043–1046 (2010)Cite this article

Abstract

In this study, three outbreaks of peste des petits ruminants (PPR) in goats and sheep flocks with high morbidity and considerable mortality were recorded at Jhansi and Revati in Uttar Pradesh and Bhopal in Madhya Pradesh, India during 2003–2006. Clinical samples were collected from the affected flocks for laboratory investigation. The PPR virus (PPRV) antigen/nucleic acid in the infected tissues/swab materials was demonstrated by using sandwich enzyme-linked immunosorbent assay (ELISA) and reverse transcription–polymerase chain reaction techniques, and the antibody to PPRV in serum samples was detected by competitive ELISA. The causative agent of the outbreaks, PPRV, was successfully isolated in Vero cells at first passage itself, and its identity was confirmed. The isolated PPR viruses belong to lineage IV based on phylogenetic analysis of partial fusion gene sequences and are closely related to other Asian or Indian PPRV isolates/strains.

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Fig. 1

Abbreviations

CPE:

Cytopathic effect

ELISA:

Enzyme-linked immunosorbent assay

MEGA 4:

Molecular evolutionary genetic analysis version 4

PBS:

Phosphate-buffered saline

PPRV:

peste des petits ruminants virus

DPI:

Days postinfection

RT–PCR:

Reverse transcription–polymerase chain reaction

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Acknowledgments

The authors thank the Director of Indian Veterinary Research Institute for providing necessary facilities to carry out this work and the staff of National Morbillivirus Referral Laboratory, IVRI, Mukteswar for their valuable and timely help in carrying out this work. The study in the form of ad hoc scheme (F.No.11-3/2007-GA-II and 1-1/2007-ASR-IV) was funded by Indian Council of Agricultural Research, Government of India, New Delhi, India.

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  1. V. Balamurugan

    Present address: Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Hebbal, Bangalore, 560 024, Karnataka, India

Authors and Affiliations

  1. Division of Virology, Indian Veterinary Research Institute, Campus Mukteswar, Nainital, 263 138, Uttarakhand, India

    V. Balamurugan, A. Sen, G. Venkatesan, V. Yadav & V. Bhanuprakash

  2. National Research Centre on Equines, Sirsa Road, Hisar, 125 001, Haryana, India

    R. K. Singh

Authors
  1. V. Balamurugan
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  2. A. Sen
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  3. G. Venkatesan
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  4. V. Yadav
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  5. V. Bhanuprakash
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  6. R. K. Singh
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Correspondence to V. Balamurugan.

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Balamurugan, V., Sen, A., Venkatesan, G. et al. Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India. Trop Anim Health Prod 42, 1043–1046 (2010). https://doi.org/10.1007/s11250-010-9527-0

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  • DOI: https://doi.org/10.1007/s11250-010-9527-0

Keywords

  • PPR
  • Outbreaks
  • Isolation
  • ELISA
  • RT–PCR assays
  • Phylogenetic analysis