Skip to main content

Advertisement

SpringerLink
Log in

Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows

  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Viral isolation, polymerase chain reaction (PCR), dot blot hybridization (DBH), and indirect enzyme-linked immunosorbent assay (iELISA) were used for the diagnosis of lumpy skin disease in clinically infected, fevered, and apparently normal dairy cows. Lumpy skin disease virus (LSDV) was isolated from skin biopsies and blood samples collected from clinically infected cows in percentages of 72% and 20%, respectively. The virus recovered from blood samples collected from fevered cows in percentage of 33.3%. Both PCR and DBH detected viral DNA in 100% of skin biopsies collected from clinically infected cows whereas the detection rates in blood samples collected from clinically infected animals were 100% and 84% using PCR and DBH, respectively. Viral DNA was detected in blood samples collected from fevered cows using PCR and DBH in percentages of 77.8% and 66.6%, respectively. Only 19.1% of blood samples collected from in-contact cows was positive for both of PCR and DBH. Detection rates of antibodies against LSDV using iELISA in serum samples collected from clinically infected and fevered cows were 56% and 11.1%, respectively, whereas all in-contact cows had no antibodies against the virus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Ali, A.A., Esmat, M., Attia, H., Selim, A. and Abdel-Hamid, Y.M., 1990. Clinical and pathological studies on lumpy skin disease in Egypt. The Veterinary Record, 127, 549-550.

    CAS  PubMed  Google Scholar 

  • Babiuk, S., Bowden, T.R., Parkyn, G., Dalman, B., Manning, L., Neufeld, J., Embury-Hyatt, C., Copps, J. and Boyle, D.B., 2008. Quantification of Lumpy skin disease virus following experimental infection in cattle. Transboundary and Emerging Diseases, 55, 299-307.

    Article  CAS  PubMed  Google Scholar 

  • Barnard, B.J., Munz, E., Dumbell, K. and Prozesky, L., 1994. Lumpy skin disease. In: J.A.W. Coetzer, G.R. Thomson, R.C. Tustin and N.P.J. Kriek (eds.), Infectious diseases of livestock with special reference to Southern Africa, Vol. 1. Oxford University Press, Cape Town. pp 604-612.

    Google Scholar 

  • Bhanuprakash, V., Hosamani, M., Juneja, S., Kumar, N. and Singh, R.K., 2006. Detection of Goat pox Antibodies: Comparative Efficacy of Indirect ELISA and Counter immunoelectrophoresis. Journal of Applied Animal Research, 30, 177-180.

    CAS  Google Scholar 

  • Bowden, T.R., Babiuk, S.L., Parkyn, G.R., Copps, J.S., Boyle, D.B., 2008. Capripoxvirus tissue tropism and shedding: A quantitative study in experimentally infected sheep and goats. Virology, 371, 380-393

    Article  CAS  PubMed  Google Scholar 

  • Carn, V.M. and Kitching, R.P., 1995. The clinical response of cattle experimentally infected with lumpy skin disease (Neethling) virus. Archive of Virology, 140, 503-513.

    Article  CAS  Google Scholar 

  • Davies, F.G., 1991. Lumpy skin disease of cattle A growing problem in Africa and the Near East. In: R.D.S. Branckaert, R. Tucker, N. Roland, T. Gumprecht, M. Criscuolo, B. Temple-Benvenuti, E.P. Cunningham, V. Kouba, A.W. Qureshi, J. Phelan, E. Lynnerup, and K. Richmond (eds.), World Animal Review, Issue number 68, 37-42

  • Davies, F.G., Krauss, H., Lund L.J. and Taylor, M., 1971. The laboratory diagnosis of lumpy skin disease. Research in Veterinary Science, 12, 123-127.

    CAS  PubMed  Google Scholar 

  • Holtke, H.J., Ankenbauer, W., Muhlegger, K., Rein, R., Sagner, G., Seibl, R. and Walter, T., 1995. The digoxigenin (DIG) system for non-radioactive labeling and detection of nucleic acids-an overview. Cell Mol Biol. 41, 883-905.

    CAS  PubMed  Google Scholar 

  • Hosamani, M., Nandi, S., Mondal, B., Singh, R.K., Rasool, T.J. and Bandyopadhyay, S.K., 2004. A Vero cell-attenuated goatpox virus provides protection against virulent virus challenge. Acta virologica, 48, 15-21.

    CAS  PubMed  Google Scholar 

  • Ireland, D.C. and Binepal, Y.S., 1998. Improved detection of capripoxvirus in biopsy samples by PCR. Journal of Virological Methods, 74, 1-7.

    Article  CAS  PubMed  Google Scholar 

  • Irons, P.C., 2007. The bull as a source of trichomonosis and lumpy skin disease: An African perspective. Ph.D. thesis, Faculty of Veterinary Medicine, University of Utrecht, Netherlands.

  • Irons, P.C., Tuppurainen, E.S.M. and Venter, E.H., 2005. Excretion of lumpy skin disease virus in bull semen. Theriogenology, 63, 1290-1297.

    Article  CAS  PubMed  Google Scholar 

  • Khandijan, E.W., 1987. Optimized hybridization of DNA blotted and fixed to nitrocellulose and nylon membranes. Bio/Technology, 5, 165.

    Article  Google Scholar 

  • Kitching, R.P. and Hammond, J.M., 1992. Poxvirus, infection and immunity. In: I.M. Roitt and P.J. Delves (eds.), Encyclopedia of immunology, Vol. 3, 3rd ed., Academic press, London. pp 1261-1264.

    Google Scholar 

  • Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K., Bakandritsos, N. and Papadopoulos O., 1999. Sheep poxvirus identification by PCR in cell cultures. Journal of Virological Methods, 77, 75-79.

    Article  CAS  PubMed  Google Scholar 

  • Mangana-Vougiouka, O., Markoulatos, P., Koptopoulos, G., Nomikou, K., Bakandritsos, N. and Papadopoulos, O., 2000. Sheep poxvirus identification from clinical specimens by PCR, cell culture, immunofluorescence and agar gel immunoprecipitation assay. Molecular and Cellular Probes, 14, 305-310.

    Article  CAS  PubMed  Google Scholar 

  • Markoulatos, P., Mangana-Vougiouka, O., Koptopoulos, G., Nomikou, K. and Papadopoulos, O., 2000. Detection of sheep poxvirus in skin biopsy samples by a multiplex polymerase chain reaction. Journal of Virological Methods, 84, 161-167.

    Article  CAS  PubMed  Google Scholar 

  • Martin, S.W., 1977. The evaluation of tests. Canadian Journal of Comparative Medicine, 41, 19-25.

    CAS  PubMed  Google Scholar 

  • Mathews, R.E.F., 1982. Classification and nomenclature of viruses. Intervirology, 17, 1-199.

    Article  Google Scholar 

  • OIE, 2008. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Office International des Epizooties, Paris, France: World Organization for Animal Health.

    Google Scholar 

  • Pandey, K.D., Rai, A., Goel, A.C., Mishra, S.C. and Gupta, B.K., 1985. Adaptation and growth cycle of sheep poxvirus in MDBK cell line. Indian Journal of Virology, 1, 133-8.

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular cloning: A laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.

    Google Scholar 

  • Southern, E.M., 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98, 503-517.

    Article  CAS  PubMed  Google Scholar 

  • Tuppurainen, E.S.M., 2004. The detection of lumpy skin disease virus in samples of experimentally infected cattle using different diagnostic techniques. M.V.Sc. thesis, Faculty of Veterinary Science, University of Pretoria, South Africa.

    Google Scholar 

  • Tuppurainen, E.S.M., Venter, E.H. and Coetzer, J.A.W., 2005. The detection of lumpy skin disease virus in samples of experimentally infected cattle using different diagnostic techniques. Onderstepoort Journal of Veterinary Research, 72, 153-164.

    CAS  PubMed  Google Scholar 

  • Wallace, D.B. and Viljoen, G.J., 2005. Immuno responses to recombinants of the South African vaccine strain of lumpy skin disease virus generated by using thymidine kinase gene insertion. Vaccine, 23, 3061-3067.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt

    Walid S. Awad

  2. Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

    Adel K. Ibrahim & Khaled Mahran

  3. Department of Clinical Pathology, Faculty of Veterinary Medicine, Banha University, Giza, Egypt

    Khaled M. Fararh

  4. Virology Department, Animal Health Research Institute, Dokki, Giza, Egypt

    Mervet I. Abdel Moniem

Authors
  1. Walid S. Awad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Adel K. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Khaled Mahran
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Khaled M. Fararh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mervet I. Abdel Moniem
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Walid S. Awad.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Awad, W.S., Ibrahim, A.K., Mahran, K. et al. Evaluation of different diagnostic methods for diagnosis of Lumpy skin disease in cows. Trop Anim Health Prod 42, 777–783 (2010). https://doi.org/10.1007/s11250-009-9486-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-009-9486-5

Keywords

Search

Navigation