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Detection of camel pox and vaccinia viruses by polymerase chain reaction

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Abstract

PCR following two methods of DNA extraction was used to confirm the growth of camel pox virus (CPV) and vaccinia virus in cell culture and chorioallantoic membrane. Results were compared with the commonly used neutralization test. The first method of DNA extraction was accomplished by using viral DNA in tissue culture supernatant and Chorioallantoic membrane, which was released by initial heating for 15 min at 99°C followed by ordinary PCR. In the second method DNA was extracted by using DNA Isolation Kit from tissue culture supernatant and used as a template. Rapid identification and differentiation of CPV and Vaccinia virus were achieved by PCR and this assay proved to be fast and feasible, and can be an alternative to orthodox serological methods.

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Abbreviations

ATI:

Acidophilic-type inclusion body

CAM:

Chorioallantoic membrane

CPE:

Cytopathic effect

CPV:

Camel pox virus

DNA:

Deoxyribonucleic acid

ELISA:

Enzyme- linked immunosorbent assay

HA:

Hem agglutinin

PCR:

Polymerase chain reaction

TCID/50:

Tissue culture infected dose 50%

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Acknowledgment

I would like to thank my colleagues in The Central Veterinary Research Laboratory at Soba, in particular Dr. Ehsan Hussin for her help and provision of some essential materials.

I am extremely grateful to the staff of the Department of Microbiology, Faculty of Vet. Medicine, University of Khartoum for providing facilities to conduct this work. My gratitude is also extended to all staff of the Virology Laboratory.

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Authors and Affiliations

  1. Department of Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science &Technology, P.O. Box: 204, Khartoum North, Sudan

    Hanan M. Sheikh Ali

  2. Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, 13311, Khartoum North, Sudan

    A. I. Khalafalla & A. H. Nimir

Authors
  1. Hanan M. Sheikh Ali
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  2. A. I. Khalafalla
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  3. A. H. Nimir
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Correspondence to Hanan M. Sheikh Ali.

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Sheikh Ali, H.M., Khalafalla, A.I. & Nimir, A.H. Detection of camel pox and vaccinia viruses by polymerase chain reaction. Trop Anim Health Prod 41, 1637–1641 (2009). https://doi.org/10.1007/s11250-009-9359-y

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  • DOI: https://doi.org/10.1007/s11250-009-9359-y

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