Skip to main content
SpringerLink
Log in

Protective antibody response produced by the chickens vaccinated with green coloured thermostable Newcastle disease virus

  • Original Paper
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

The efficacy of green-coloured (GC) I-2 Newcastle disease vaccine was determined in the present study. I-2 vaccine was mixed with a green coloured dye and stored at 4°C for 6 months while assayed for the virus infectivity at a monthly interval. Chickens were vaccinated with the GC vaccine by eye drop. Serum samples were collected from all birds before and after vaccination at weekly interval for 4 weeks and tested for haemagglutination-inhibition (HI) antibody against Newcastle disease virus (NDV). These chickens were challenged with NDV virulent strain four weeks after vaccination. The results showed that there was no difference between the infectivity titres of GC and uncoloured vaccines. However, chickens vaccinated with GC vaccine produced higher HI antibody titres than chickens vaccinated with uncoloured vaccine. Results from the challenge trial showed that all vaccinated chickens survived whereas all unvaccinated chickens died. The findings from this study have shown that the GC vaccine is safe and produced protective antibodies against NDV in vaccinated chickens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EID50 :

median embryo infectious dose

HA:

haemagglutination

HI:

haemagglutination-inhibition

NDV:

Newcastle disease virus

References

  • Alexander, D. J., 1998. Newcastle disease. In: D. E. Swayne, J. R. Glisson, M. W. Jackwood, J. E. Pearson, W. M. Reed, (eds.), A laboratory manual for isolation and identification of avian pathogens, 4th edn., (American Association of Avian Pathologists, Kennett Square, PA), 156–163

    Google Scholar 

  • Amakye-Anim, J., Awuni, J.A., Coleman, T., and Seddor, V., 2000. Ghanaian trials with a locally-produced thermostable Newcastle disease vaccine (strain I-2) in chickens. 26th Animal Science Symposium, Ghana Animal Science Association. Kumasi, University of Science and Technology.

    Google Scholar 

  • Bensink, Z., and Spradbrow P., 1999. Newcastle disease virus strain I-2-a prospective thermostable vaccine for use in developing countries. Veterinary Microbiology, 68, 131–139

    Article  PubMed  CAS  Google Scholar 

  • Cargill, P., 1999. Vaccine administration in poultry. In Practice, 21, 323–328

    Google Scholar 

  • Dias, P.T., Alders, R.G., Fringe, R. and Mata, B.V., 2001. Laboratory and field trials with thermostable live Newcastle disease vaccines in Mozambique. In: R. G. Alders and P. B. Spradbrow, (eds.), SADC Planning Workshop on Newcastle Disease Control in Village Chickens. ACIAR Proceedings, 103, 91–96

  • Meulemans, G., 1988. Control by vaccination. In: D. J. Alexander (ed.), Newcastle Disease, (Kluwer Academic Publications, Boston), 318–332

    Google Scholar 

  • Reed, L. S., Muench, L. H., 1938. A simple method of estimating fifty percent endpoints. American Journal Hygiene, 27, 493–497

    Google Scholar 

  • SAS Institute Inc., 1986. Language Guide for Personal Computers. Version 6. Cary NC: SAS Institute Inc.

    Google Scholar 

  • Spradbrow, P.B., 1993/94. Newcastle disease in village chickens. Poultry Science Review, 5, 57–96.

    Google Scholar 

  • Spradbrow, P.B. and Copland, J.W., 1996. Production of thermostable Newcastle disease virus in developing countries. Preventive Veterinary Medicine 29, 157–159

    Article  Google Scholar 

  • Spradbrow, P.B., Mackenzie, M. and Grimes, S.E., 1995. Recent isolates of Newcastle disease virus. Veterinary Microbiology, 46, 21–28

    Article  PubMed  CAS  Google Scholar 

  • Thorntorn, D. H., 1988. Quality control of vaccines. In: D. J. Alexander (ed.) Newcastle Disease, (Kluwer Academic Publishers, Boston), 347–365

    Google Scholar 

  • Tu, T.D., Phuc, K. V., Dihn, N.T.K., Quoc, D.N. and Spradbrow, P.B., 1998. Vietnamese trials with a thermostable Newcastle disease vaccine (Strain I-2) in experimental and village chickens. Preventive Veterinary Medicine, 34, 205–214

    Article  PubMed  CAS  Google Scholar 

  • Wambura, P. N., A. M. Kapaga and Hyera, J.M.K., 2000. Experimental trials with a thermostable Newcastle disease virus (strain I-2) in commercial and village chickens in Tanzania. Preventive Veterinary Medicine, 43, 75–83

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology and Parasitology, Sokoine University of Agriculture, P. O. Box 3019, Chuo Kikuu, Morogoro, Tanzania

    P. N. Wambura

Authors
  1. P. N. Wambura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to P. N. Wambura.

Additional information

Wambura, P. N., 2008. Protective antibody response produced by the chickens vaccinated with green coloured thermostable Newcastle disease virus. Tropical Animal Health and Production.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wambura, P.N. Protective antibody response produced by the chickens vaccinated with green coloured thermostable Newcastle disease virus . Trop Anim Health Prod 41, 149–152 (2009). https://doi.org/10.1007/s11250-008-9169-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-008-9169-7

Keywords

Search

Navigation