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Degradation of transgenic Bacillus thuringiensis proteins in corn tissue in response to post-harvest management practices

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Abstract

Knowledge of the persistence of Cry proteins in transgenic corn residue after harvest is necessary to assess the ecological risk to nontarget organisms. The amount of protein remaining in crop residue declines over time by a combination of microbial decomposition and leaching, both influenced by temperature, precipitation, and the amount of residue-soil contact. Here, we investigated how long biologically active Cry proteins persist in SmartStax corn residue expressing Cry1A.105, Cry1F, Cry2Ab2, Cry3Bb1, and Cry34/35Ab1, when subjected to four post-harvest practices (chisel plow tillage, flail mowing, cover crop planting, and undisturbed residue). Protein activity in residue samples collected up to 25 weeks after harvest was measured by Ostrinia nubilalis feeding bioassays and cross validated with detection frequencies determined by ELISA. All corn residue remained above ground in the flail-mowed and undisturbed treatments, while the cover crop and chisel plow treatments left 88.3 and 39.6% of the residue remaining above ground, respectively. Cry proteins retained biological activity for as long as 24 weeks after harvest when residue was left above ground with less soil contact, typical of no-till corn systems. ELISA detections were positively correlated with results of the feeding bioassays, which revealed the presence of active proteins beyond the point of ELISA detection.

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Acknowledgements

We are grateful to T. Patton, M. Dimock and E. Zobel for their assistance in the field and laboratory experimentation and data collection. We would also like to thank the field crew at the Central Maryland Research and Education Center in Beltsville, Maryland for their time, patience, and expert technical assistance.

Funding

This work was supported by a USDA NIFA Extension Integrated Pest Management Coordination and Support Program (EIPM-CS) award number 2013-41534-21512, and USDA NIFA Biotechnology Risk Assessment Grant Program (BRAG) award number 2014-33522.

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VY, GPD, WOL and CRRH designed the experiments. VY and GPD performed the experiments, and GPD analyzed the data. VY, GPD and AL wrote the manuscript, and WOL and CRRH edited the manuscript.

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Correspondence to V. Yurchak.

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We do not have any conflicting financial, personal, or professional interests that are related to this manuscript.

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Yurchak, V., Leslie, A.W., Dively, G.P. et al. Degradation of transgenic Bacillus thuringiensis proteins in corn tissue in response to post-harvest management practices. Transgenic Res 30, 851–865 (2021). https://doi.org/10.1007/s11248-021-00273-8

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  • DOI: https://doi.org/10.1007/s11248-021-00273-8

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