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Association between sheath blight resistance and chitinase activity in transgenic rice plants expressing McCHIT1 from bitter melon

Abstract

No usable resources with high-level resistance to sheath blight (SB) have yet been found in rice germplasm resources worldwide. Therefore, creating and breeding new disease-resistant rice resources with sheath blight resistance (SBR) are imperative. In this study, we inoculated rice plants with hyphae of the highly pathogenic strain RH-9 of rice SB fungus Rhizoctonia solani to obtain eight stable transgenic rice lines harbouring the chitinase gene (McCHIT1) of bitter melon with good SBR in the T5 generation. The mean disease index for SB of wild-type plants was 92% and 37–44% in transgenic lines. From 24 h before until 120 h after inoculation with R. solani, chitinase activity in stable transgenic plants with increased SBR was 2.0–5.5 and 1.8–2.7 times that of wild-type plants and plants of a disease-susceptible stable transgenic line, respectively. The correlation between SBR and chitinase activity in McCHIT1-transgenic rice line plants was significant. This work stresses how McCHIT1 from bitter melon can be used to protect rice plants from SB infection.

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Abbreviations

ANOVA:

Analysis of variance

BSA:

Bovine serum albumin

GlcNAc:

N-acetylglucosamine

GUS:

β-Glucuronidase

NaAc:

Sodium acetate

PMSF:

Pheylmethylsulfonyl fluoride

PR:

Pathogenesis-related gene

SB:

Sheath blight

SBR:

Sheath blight resistance

SSR:

Shortest significant ranges

X-Gluc:

5-Bromo-4-chloro-3-indolyl glucuronide

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Acknowledgements

The study was funded by Major Projects of National New Transgenic Species Breeding (2016ZX08001-002), General Projects of Technological Innovation and Application Demonstration (Social Livelihood) in Chongqing City (cstc2018jscx-msybX0250), and PhD Funds (including the Talent Introduction Plan) of Southwest University (Project SWU112077).

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Correspondence to Guanghua He.

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Changwei Zhang and Manyu Huang have equally contributed

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Zhang, C., Huang, M., Sang, X. et al. Association between sheath blight resistance and chitinase activity in transgenic rice plants expressing McCHIT1 from bitter melon. Transgenic Res 28, 381–390 (2019). https://doi.org/10.1007/s11248-019-00158-x

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  • DOI: https://doi.org/10.1007/s11248-019-00158-x

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