Transgenic Research

, Volume 25, Issue 5, pp 561–573

CRISPR/Cas9: an advanced tool for editing plant genomes

  • Milan Kumar Samanta
  • Avishek Dey
  • Srimonta Gayen

DOI: 10.1007/s11248-016-9953-5

Cite this article as:
Samanta, M.K., Dey, A. & Gayen, S. Transgenic Res (2016) 25: 561. doi:10.1007/s11248-016-9953-5


To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.


Sequence-specific nucleases Genome editing CRISPR/Cas9 TALENs ZFNs Plants 

Funding information

Funder NameGrant NumberFunding Note
Indian Council of Agricultural Research
    Department of Science and Technology, INDIA

      Copyright information

      © Springer International Publishing Switzerland 2016

      Authors and Affiliations

      • Milan Kumar Samanta
        • 1
      • Avishek Dey
        • 1
      • Srimonta Gayen
        • 2
      1. 1.Advanced Laboratory for Plant Genetic EngineeringIndian Institute of TechnologyKharagpurIndia
      2. 2.Department of Human GeneticsUniversity of MichiganAnn ArborUSA

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