Transgenic Research

, Volume 26, Issue 2, pp 225–235

Citrus psorosis virus coat protein-derived hairpin construct confers stable transgenic resistance in citrus against psorosis A and B syndromes

Original Paper

DOI: 10.1007/s11248-016-0001-2

Cite this article as:
De Francesco, A., Costa, N. & García, M.L. Transgenic Res (2017) 26: 225. doi:10.1007/s11248-016-0001-2
  • 99 Downloads

Abstract

Citrus psorosis virus (CPsV) is the causal agent of psorosis, a serious and widespread citrus disease. Two syndromes of psorosis, PsA and PsB, have been described. PsB is the most aggressive and rampant form. Previously, we obtained Pineapple sweet orange plants transformed with a hairpin construct derived from the CPsV coat protein gene (ihpCP). Some of these plants were resistant to CPsV 90-1-1, a PsA isolate homologous to the transgene. In this study, we found that expression of the ihpCP transgene and siRNA production in lines ihpCP-10 and -15 were stable with time and propagation. In particular, line ihpCP-15 has been resistant for more than 2 years, even after re-inoculation. The ihpCP plants were also resistant against a heterologous CPsV isolate that causes severe PsB syndrome. Line ihpCP-15 manifested complete resistance while line ihpCP-10 was tolerant to the virus, although with variable behaviour, showing delay and attenuation in PsB symptoms. These lines are promising for a biotech product aimed at eradicating psorosis.

Keywords

Citrus psorosis virus Hairpin-RNA Psorosis A Psorosis B Transgenic sweet orange Virus resistance 

Supplementary material

11248_2016_1_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 kb)
11248_2016_1_MOESM2_ESM.docx (29 kb)
Supplementary material 2 (DOCX 28 kb)

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Instituto de Biotecnología y Biología Molecular, CCT-La PlataCONICET - UNLPLa PlataArgentina
  2. 2.Estación Experimental AgropecuariaINTA ConcordiaConcordiaArgentina