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Transgenic Research

, Volume 24, Issue 6, pp 945–953 | Cite as

Increased production of wax esters in transgenic tobacco plants by expression of a fatty acid reductase:wax synthase gene fusion

  • Selcuk Aslan
  • Per Hofvander
  • Paresh Dutta
  • Chuanxin Sun
  • Folke SitbonEmail author
Original Paper

Abstract

Wax esters are hydrophobic lipids consisting of a fatty acid moiety linked to a fatty alcohol with an ester bond. Plant-derived wax esters are today of particular concern for their potential as cost-effective and sustainable sources of lubricants. However, this aspect is hampered by the fact that the level of wax esters in plants generally is too low to allow commercial exploitation. To investigate whether wax ester biosynthesis can be increased in plants using transgenic approaches, we have here exploited a fusion between two bacterial genes together encoding a single wax ester-forming enzyme, and targeted the resulting protein to chloroplasts in stably transformed tobacco (Nicotiana benthamiana) plants. Compared to wild-type controls, transgenic plants showed both in leaves and stems a significant increase in the total level of wax esters, being eight-fold at the whole plant level. The profiles of fatty acid methyl ester and fatty alcohol in wax esters were related, and C16 and C18 molecules constituted predominant forms. Strong transformants displayed certain developmental aberrations, such as stunted growth and chlorotic leaves and stems. These negative effects were associated with an accumulation of fatty alcohols, suggesting that an adequate balance between formation and esterification of fatty alcohols is crucial for a high wax ester production. The results show that wax ester engineering in transgenic plants is feasible, and suggest that higher yields may become achieved in the near future.

Keywords

Fatty acyl reductase Tobacco (Nicotiana benthamianaTransgenic plants Wax ester Wax synthase 

Notes

Acknowledgments

We thank Prof. Sten Stymne (Dept. of Plant Breeding, SLU, Alnarp) for support and advice, and Drs. Sarosh Bejai (Dept. of Plant Biology, SLU, Uppsala) and Frédéric Domergue (Laboratoire de Biogenèse Membranaire, CNRS, Univ. Bordeaux Ségalen, Bordeaux, France) for advice concerning QPCR data analysis and DNA constructions, respectively. The work was supported by EU FP7 project “ICON”, the Swedish Research Council Formas, and the Swedish Governmental Agency for Innovation Systems, VINNOVA.

Conflict of interest

The authors declare they have no competing interests.

Supplementary material

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Selcuk Aslan
    • 1
  • Per Hofvander
    • 2
  • Paresh Dutta
    • 3
  • Chuanxin Sun
    • 1
  • Folke Sitbon
    • 1
    Email author
  1. 1.Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant BiologySwedish University of Agricultural Sciences (SLU)UppsalaSweden
  2. 2.Department of Plant BreedingSwedish University of Agricultural Sciences (SLU)AlnarpSweden
  3. 3.Department of Food ScienceSwedish University of Agricultural Sciences (SLU)UppsalaSweden

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