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Transgenic Research

, Volume 24, Issue 5, pp 859–873 | Cite as

Ectopic expression of GroEL from Xenorhabdus nematophila in tomato enhances resistance against Helicoverpa armigera and salt and thermal stress

  • Punam Kumari
  • Gagan Kumar Mahapatro
  • Nirupama BanerjeeEmail author
  • Neera Bhalla SarinEmail author
Original Paper

Abstract

The GroEL homolog XnGroEL protein of Xenorhabdus nematophila belongs to a highly conserved family of molecular chaperones/heat shock proteins (Hsps). XnGroEL was shown to possess oral insecticidal activity against a major crop pest Helicoverpa armigera. Under normal conditions, the Hsps/chaperones facilitate folding, assembly, and translocation of cellular proteins, while in stress conditions they protect proteins from denaturation. In this study, we describe generation of transgenic tomato plants overexpressing insecticidal XnGroEL protein and their tolerance to biotic and abiotic stresses. Presence of XnGroEL in the transgenic tomato lines conferred resistance against H. armigera showing 100 % (p ≤ 0.001) mortality of neonates. In addition, XnGroEL provided thermotolerance and protection against high salt concentration to the tomato plants. Expression of XnGroEL minimized photo-oxidation of chlorophyll and reduced oxidative damage of cell membrane system of the plants under heat and salt stress. The enhanced tolerance to abiotic stresses correlated with increase in the anti-oxidative enzyme activity and reduced H2O2 accumulation in transgenic tomato plants. The variety of beneficial properties displayed by XnGroEL protein provides an opportunity for value addition and improvement of crop productivity.

Keywords

Helicoverpa armigera Heat stress Insect resistance Insecticidal XnGroEL Salt stress Xenorhabdus nematophila 

Notes

Acknowledgments

The Grant from the Department of Biotechnology (No. BT/PR11260/PBD/16/819/2008) to Prof. Neera Bhalla Sarin and Dr. Nirupama Banerjee is gratefully acknowledged. P. K. acknowledges the financial support from the Council for Scientific and Industrial Research. Partial funds from Department of Science and Technology (D.S.T.-PURSE, D.S.T.-F.I.S.T.), U.G.C.-C.A.S., U.G.C.-R.N.W., and J.N.U are gratefully acknowledged.

Supplementary material

11248_2015_9881_MOESM1_ESM.ppt (53 kb)
Supplementary material 1 (PPT 53 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia
  2. 2.Division of EntomologyIndian Agricultural Research InstituteNew DelhiIndia

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