Abstract
Based on farm field plot level survey data and laboratory test, we examine the determinants of the expression of Bt toxin in China’s Bt cotton production. The results show that the expression of Bt toxin differs significantly among varieties. Even for the same variety the expression of Bt toxin also varies substantially among villages and among farmers in the same village. Econometric analyses show that after controlling for the effects of varieties and locations (or villages), farm management, particular applications of phosphate and potash fertilizers, and manure, has significant positive effects on Bt toxin expression in farmer’s fields. In contrast to previous studies which showed that nitrogen fertilizer has a positive impact on expression of Bt toxin, this study shows that nitrogen fertilizer has no significant impact on expression of Bt toxin in farmer’s fields. On the other hand, the expression of Bt toxin has a positive relationship with phosphate fertilizer, potash fertilizer and manure application.
This is a preview of subscription content, access via your institution.


Notes
Xinjiang Uyghur Autonomous Region is the largest cotton production region in China. However, because of the hot and dry climate, the cotton bollworm is not a serious problem in Xinjiang.
References
Adamczyk J, Sumerford D (2001) Potential factors impacting season-long expression of Cry1Ac in 13 commercial varieties of Bollgard cotton. J Insect Sci 1:1–6
Adamczyk J, Hardee D, Adams L, Sumerford D (2001) Correlating differences in larval survival and development of bollworm (Lepidopera: Noctuidae) and fall armyworm (Lepidoptera: Noctuidae) to differential expression of Cry1Ac deltaendotoxin in various plant parts among commercial cultivars of transgenic Bacillus thuringiensis cotton. J Econ Entomol 94:284–290
Baute T (2004) A Grower’s handbook: controlling corn insect pests with Bt corn technology, 2nd edn. Canadian Corn Coalition, Canada
Carlos E, Robert D, John T (2002) Plant allocation to defensive compounds: interactions between elevated CO2 and nitrogen in transgenic cotton plants. J Exp Bot 53:323–331
Coviella C, Stipanovic R, Trumble J (2002) Plant allocation to defensive compounds: interactions between elevated CO2 and nitrogen in transgenic cotton plants. J Exp Bot 53:323–331
Cui J, Xia J (1999) The spatio-temporal distribution of Bt (Bacillus thuringiensis) insecticidal protein in Bt cotton. Cott Sci 11(3):141–146
FAO (2001) Action against undernutrition and poverty. Redirecting food assistance to those who need it most, 2001. In: The state of food insecurity in the World 2001, ISBN 92-5-104628-X. http://www.fao.org/docrep/003/y1500e/y1500e05.htm#P0_0. Accessed 6 June 2011
Gould F (1998) Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology. Annu Rev Entomol 43:701–726
Greenplate J (1999) Quantification of Bacillus thuringiensis insect control protein Cry1Ac over time in Bollgard cotton fruit and terminals. J Econ Entomol 92:1377–1383
Greenplate J, Penn S, Mullins J, Oppenhuizen M (2000) Seasonal Cry1Ac levels in DP50B: the Bollgard basis for Bollgard II. In: Dugger P, Richter R (eds) Proceedings of the Beltwide cotton conference, San Antonio, TX, 4–8 Jan. 2000, pp 1039–1041. National Cotton Council of America, Memphis, TN
Greenplate J, Mullins W, Penn S, Embry K (2001) Cry1Ac Bollgard varieties as influenced by environment, variety and plant age: 1999 gene equivalency field studies. In: Dugger P, Richter R (eds) Proceedings of the Beltwide cotton conference, vol 2. National Cotton Council of America, Memphis, TN, pp 790–793
Gutierrez A, Adamczyk J, Ponsard S, Ellis C (2006) Physiologically based demographics of Bt cotton–pest interactions II. Temporal refuges, natural enemy interactions. Ecol Model 191:360–382
Holt H (1998) Season-long monitoring of transgenic cotton plants-development of an assay for the quantification of Bacillus thuringiensis insecticidal protein. In: Proceedings 9th Australian cotton grower research association. Queensland, Wee Waa, Australia, pp 331–335
Huang J, Rozelle S, Pray C, Wang Q (2002) Plant biotechnology in China. Science 295(25):674–677
Huang J, Hu R, Pray C, Qiao F, Rozelle S (2003) Biotechnology as an alternative to chemical pesticides: a case study of Bt cotton in China. Agr Econ 29:55–67
Huang J, Hu R, Cao J, Rozelle S (2008) Training programs and in the field guidance to reduce China’s overuse of fertilizer without hurting profitability. J Soil Water Conserv 63(5):165–167
James C (2012) Global status of commercialized Biotech/GM Crops: 2012. ISAAA Brief No. 44. ISAAA, Ithaca, NY
Kranthi K, Naidu S, Dhawad C, Tatwawadi A, Mate K, Patil E, Bharose A, Behere G, Wadaskar R, Kranthi S (2005) Temporal and intraplant variability of Cry1Ac expression in Bt-cotton and its influence on the survival of the cotton bollworm, Helicoverpa armigera. Curr Sci India 89:291–298
Mahon R, Finnergan J, Olsen K, Lawrence L (2002) Environmental stress and the efficacy of Bt cotton. Aust Cotton Grow 22:18–21
National Bureau of Statistics of China (2008) China statistics yearbook 2008. China Statistical Express, Beijing
Pray C, Ma D, Huang J, Qiao F (2001) Impact of Bt cotton in China. World Dev 29:813–825
Qaim M (2003) Bt cotton in India: field trial results and economic projections. World Dev 31(12):2115–2127
Qiao F, Wilen J, Rozelle S (2008) Dynamically optimal strategy to manage resistance to genetically modified (GM) crops. J Econ Entomol 101:915–926
Qiao F, Huang J, Rozelle S, Wilen J (2010) Natural refuge crops, buildup of resistance, and zero-refuge strategy for Bt cotton in China. Sci China Life Sci 53(10):1–12
Rao C (2005) Transgenic Bt technology: 3. Expression of transgenes. http://www.monsanto.co.uk/news/ukshowlib.php?uid=9304. Accessed 6 June 2011
Sachs E, Benedict J, Stelly D, Taylor J, Altman D, Berberich S, Daivs S (1998) Expression and segregation of genes encoding CryIA insecticidal proteins in cotton. Crop Sci 38(1):1–11
Shen P, Lin K, Zhang Y, Wu K, Guo Y (2010) Seasonal expression of Bacillus thuringiensis insecticidal protein and the control to cotton bollworm in different varieties of transgenic cotton. Cotton Sci 22(5):393–397
Wang R, Liu A, Li R (1997) Bt cotton R93-6 and nitrogen fertilizer requirement. Cotton Sci 24(4):16–17
Wen S, Dong H, Xin C (2007) Research progress in differential expression of Bt efficacy in transgenic cotton. J Henan Agric Sci 1:9–13
Xing C, Jing S, Cui X, Guo L, Wang H, Yuan Y (2001) The spatio-temporal distribution of Bt (Bacillus thuringiensis) insecticidal protein and the effect of transgenic Bt cotton on bollworm resistance. Cotton Sci 13(1):11–15
Yang C, Xu L, Yang D (2005) Effects of nitrogen fertilizer on the Bt-protein content in transgenic cotton and nitrogen metabolism mechanism. Cotton Sci 17(4):227–231
Zhang Y, Wu K, Guo Y (2001) On the spatio-temporal expression of the contents of Bt insecticidal protein and the resistance of Bt transgenic cotton to cotton bollworm. Acta Phytophy Sin 8(1):1–6
Zhang L, Huang J, Qiao F, Rozelle S (2006) Economic evaluation and analysis of fertilizer overuse by China’s farmers. In: Zhu Z, Norse D, Sun B (eds) Policy for reducing non-point pollution from crop production in China. Environment Science Press of China, Beijing
Zhou D, Wu Z, Wang X, Ni C, Zheng H, Xia J (2000) Influence of fertilization and environmental temperature on the resistance of Bt transgenic cotton to cotton bollworm. J Anhui Agric Univ 27(4):352–357
Acknowledgments
The authors are grateful to the staff of the Center for Chinese Agricultural Policy who worked so hard in collecting data. We would also like to thank Carl Pray, Scott Rozelle, Robert Tripp, and Ellie Osir for their comments. Additionally, the authors acknowledge the financial supports of this study from the GMO Program of the Ministry of Agriculture (2011ZX08015-002A), National Natural Science Foundation of China (71273290), and International Development Research Center (IDRC).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Huang, J., Mi, J., Chen, R. et al. Effect of farm management practices in the Bt toxin production by Bt cotton: evidence from farm fields in China. Transgenic Res 23, 397–406 (2014). https://doi.org/10.1007/s11248-013-9775-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11248-013-9775-7