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Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease

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Abstract

After several decades of research, autosomal dominant polycystic kidney disease (ADPKD) is still incurable and imposes enormous physical, psychological, and economic burdens on patients and their families. Murine models of ADPKD represent invaluable tools for studying this disease. These murine forms of ADPKD can arise spontaneously, or they can be induced via chemical or genetic manipulations. Although these models have improved our understanding of the etiology and pathogenesis of ADPKD, they have not led to effective treatment strategies. The mini-pig represents an effective biomedical model for studying human diseases, as the pig’s human-like physiological processes help to understand disease mechanisms and to develop novel therapies. Here, we tried to generate a transgenic model of ADPKD in pigs by overexpressing c-Myc in kidney tissue. Western-blot analysis showed that c-Myc was overexpressed in the kidney, brain, heart, and liver of transgenic pigs. Immunohistochemical staining of kidney tissue showed that exogenous c-Myc predominantly localized to renal tubules. Slightly elevated blood urea nitrogen levels were observed in transgenic pigs 1 month after birth, but no obvious abnormalities were detected after that time. In the future, we plan to subject this model to renal injury in an effort to promote ADPKD progression.

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Acknowledgments

This study was funded by the Chinese National Basic Research Program (“973” Program) (Project No. 2011CB944100).

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Authors and Affiliations

  1. State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, China

    Jianhua Ye, Jin He, Qiuyan Li, Yaofeng Zhao, Xiaoxiang Hu, Zhengquan Yu & Ning Li

  2. Department of Nephrology, State Key Laboratory of Kidney Diseases, Chinese P.L.A. General Hospital, Beijing, 100853, China

    Yuanyuan Feng, Xueyuan Bai & Xiangmei Chen

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  1. Jianhua Ye
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  2. Jin He
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  3. Qiuyan Li
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  9. Zhengquan Yu
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  10. Ning Li
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Correspondence to Ning Li.

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Jianhua Ye and Jin He contributed equally to this work.

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Ye, J., He, J., Li, Q. et al. Generation of c-Myc transgenic pigs for autosomal dominant polycystic kidney disease. Transgenic Res 22, 1231–1239 (2013). https://doi.org/10.1007/s11248-013-9707-6

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