Skip to main content
Log in

Gender non-specific efficacy of ZFN mediated gene targeting in pigs

  • Letter to the Editor
  • Published:
Transgenic Research Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

References

  • Bibikova M, Golic M, Golic KG, Carroll D (2002) Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases. Genetics 161:1169–1175

    PubMed  CAS  Google Scholar 

  • Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA, Robl JM (1998) Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256–1258

    Article  PubMed  CAS  Google Scholar 

  • Dai Y, Vaught TD, Boone J, Chen SH, Phelps CJ, Ball S, Monahan JA, Jobst PM, McCreath KJ, Lamborn AE, Cowell-Lucero JL, Wells KD, Colman A, Polejaeva IA, Ayares DL (2002) Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nat Biotechnol 20:251–255

    Article  PubMed  CAS  Google Scholar 

  • Estrada J, Sommer J, Collins B, Mir B, Martin A, York A, Petters RM, Piedrahita JA (2007) Swine generated by somatic cell nuclear transfer have increased incidence of intrauterine growth restriction (IUGR). Cloning Stem Cells 9:229–236

    Article  PubMed  CAS  Google Scholar 

  • Fujimura T, Takahagi Y, Shigehisa T, Nagashima H, Miyagawa S, Shirakura R, Murakami H (2008) Production of alpha 1,3-galactosyltransferase gene-deficient pigs by somatic cell nuclear transfer: a novel selection method for gal alpha 1,3-Gal antigen-deficient cells. Mol Reprod Dev 75:1372–1378

    Article  PubMed  CAS  Google Scholar 

  • Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jenkins SS, Wood A, Cui X, Meng X, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H, Menoret S, Anegon I, Davis GD, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jacob HJ, Buelow R (2009) Knockout rats via embryo microinjection of zinc-finger nucleases. Science 325:433

    Article  PubMed  CAS  Google Scholar 

  • Hauschild J, Petersen B, Santiago Y, Queisser AL, Carnwath JW, Lucas-Hahn A, Zhang L, Meng X, Gregory PD, Schwinzer R, Cost GJ, Niemann H (2011) Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases. Proc Natl Acad Sci USA 108:12013–12017

    Article  PubMed  CAS  Google Scholar 

  • Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y (1998) Eight calves cloned from somatic cells of a single adult. Science 282:2095–2098

    Article  PubMed  CAS  Google Scholar 

  • Mohiuddin MM, Corcoran PC, Singh AK, Azimzadeh A, Hoyt RF Jr, Thomas ML, Eckhaus MA, Seavey C, Ayares D, Pierson RN 3rd, Horvath KA (2012) B-cell depletion extends the survival of GTKO.hCD46Tg pig heart xenografts in baboons for up to 8 months. Am J Transplant 12:763–771

    Article  PubMed  CAS  Google Scholar 

  • Nottle MB, Beebe LF, Harrison SJ, McIlfatrick SM, Ashman RJ, O’Connell PJ, Salvaris EJ, Fisicaro N, Pommey S, Cowan PJ, d’Apice AJ (2007) Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer. Xenotransplantation 14:339–344

    Article  PubMed  Google Scholar 

  • Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD, Holmes MC (2005) Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 435:646–651

    Article  PubMed  CAS  Google Scholar 

  • Wakayama T, Yanagimachi R (1999) Cloning of male mice from adult tail-tip cells. Nat Genet 22:127–128

    Article  PubMed  CAS  Google Scholar 

  • Wakayama T, Perry AC, Zuccotti M, Johnson KR, Yanagimachi R (1998) Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369–374

    Article  PubMed  CAS  Google Scholar 

  • Wakayama S, Ohta H, Kishigami S, Thuan NV, Hikichi T, Mizutani E, Miyake M, Wakayama T (2005) Establishment of male and female nuclear transfer embryonic stem cell lines from different mouse strains and tissues. Biol Reprod 72:932–936

    Article  PubMed  CAS  Google Scholar 

  • Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385:810–813

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Gregory J. Cost (Sangamo Biosciences) for helpful comments on the manuscript. We also thank Edward Kufeld and the staff of the pig facility in Mariensee for preparation of the animals for surgery and perfect care-taking. Additionally, we thank Klaus-Gerd Hadeler, Rolf-Peter Poppenga and Petra Hassel for surgical assistance. This study was funded by EU project Xenome and DFG FOR 535.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Bjoern Petersen or Heiner Niemann.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hauschild-Quintern, J., Petersen, B., Queisser, AL. et al. Gender non-specific efficacy of ZFN mediated gene targeting in pigs. Transgenic Res 22, 1–3 (2013). https://doi.org/10.1007/s11248-012-9647-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11248-012-9647-6

Keywords

Navigation