Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional alleles
- 265 Downloads
Transgenic and conditional knockout mouse models play an important role in biomedical research and their use has grown exponentially in the last 5–10 years. Generating conditional knockouts often requires breeding multiple alleles onto the background of a single mouse or group of mice. Breeding these mice depends on parental genotype, litter size, transmission frequency, and the number of breeding rounds. Therefore, a well planned breeding strategy is critical for keeping costs to a minimum. However, designing a viable breeding strategy can be challenging. With so many different variables this would be an ideal task for a computer program. To facilitate this process, we created a Java-based program called Conditional Allele Mouse Planner (CAMP). CAMP is designed to provide an estimate of the number of breeders, amount of time, and costs associated with generating mice of a particular genotype. We provide a description of CAMP, how to use it, and offer it freely as an application.
KeywordsTransgenics Conditional knockout Cre Floxed Frt Flp Gfp Tet Java program Software
- Donnelly CJ, McFarland M, Ames A, Sundberg B, Springer D, Blauth P, Bult CJ (2010) JAX Colony Management System (JCMS): an extensible colony and phenotype data management system. Mamm Genome 21:205–215Google Scholar
- Institute of Laboratory Animal Resources (US) Committee on Revision of the Guide for Laboratory Animals Facilities and Care, Institute of Laboratory Animal Resources (US), Committee on the Guide for Laboratory Animal Facilities and Care, and Animal Care Panel (US), Animal Facilities Standards Committee, Guide for laboratory animal facilities and care (2006) In: Publication/Public Health Service. US Public Health Service: for sale by the Supt of Docs, BethesdaGoogle Scholar
- Milisavljevic M, Hearty T, Wong TY, Portales-Casamar E, Simpson EM, Wasserman WW (2010) Laboratory Animal Management Assistant (LAMA): a LIMS for active research colonies. Mamm Genome 21:224–230Google Scholar