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Spatial monitoring of toxicity in HMOX-LacZ transgenic mice

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Abstract

Transgenic reporter mice can contribute in the development of less toxic and more selective drugs to treat disease. In this brief communication we describe the generation and initial validation of transgenic mice that provide a visual spatial readout of oxidative stress. These mice carry a LacZ reporter transgene driven by the human haem oxygenase 1 promoter. The induction of LacZ staining by a range of compounds indicated differences in the haem oxygenase 1 spatial response within a tissue. Thus this transgene allows for the spatial monitoring of differences in toxic insult and indicates that this type of transgenic system could have use in toxicity screens.

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Acknowledgement

RY was supported by BBSRC CASE award with CXR Biosciences. This work was further supported by the BBSRC and through the European Commission NEST029025 project INTEGRA. We are grateful to the animal care staff for the assistance in this project.

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Authors and Affiliations

  1. Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, Roslin, Midlothian, EH25 9PS, UK

    Rachel Young & C. Bruce A. Whitelaw

  2. Dundee Technopole, CXR Biosciences Limited, James Lindsay Place, Dundee, DD1 5JJ, UK

    C. Roland Wolf & Ken Brown

  3. Ninewells Hospital and Medical School, University of Dundee Biomedical Research Centre, Dundee, DD1 9SY, UK

    C. Roland Wolf & John D. Hayes

  4. Génétique Animale et Biologie Intégrative, Institut National de la Recherche Agronomique, 78350, Jouy-en-Josas, France

    Rachel Young

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  1. Rachel Young
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  2. C. Roland Wolf
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  3. Ken Brown
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  4. John D. Hayes
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  5. C. Bruce A. Whitelaw
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Correspondence to Rachel Young.

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Young, R., Wolf, C.R., Brown, K. et al. Spatial monitoring of toxicity in HMOX-LacZ transgenic mice. Transgenic Res 19, 897–902 (2010). https://doi.org/10.1007/s11248-010-9363-z

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  • DOI: https://doi.org/10.1007/s11248-010-9363-z

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