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Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors

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Abstract

Dendritic cell (DC) specific transgenic mice are a most important model for investigating dendritic cell functions in vivo. Recently, lentivirus mediated gene transfer has become a powerful and convenient method for generation of transgenic mice. We cloned a 1.2 kb CD11c promoter and constructed a lentiviral vector, which efficiently drove DC-specific expression in vitro. After microinjection of purified virus into the perivitelline space of single-cell embryo, more than 80% newborn mice were transgenic and 7 F0 founders were rapidly generated in 2 months. GFP was strictly expressed in CD11c+ cells in spleens, thymus and lymph nodes of the transgenic mice. Importantly, the physiological characteristics and functions of DCs in the transgenic mice were not altered by the specific expression. These results indicate that this vector could be used to rapidly prepare DC-specific transgenic mice. Thus, this lentiviral vector system may provide a convenient and useful tool to study the properties of DCs in vivo.

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Abbreviations

BM DCs:

Bone marrow derived dendritic cells

TFBS:

Transcriptional factor binding site

shRNA:

Small hairpin RNA

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Acknowledgments

We thank Dr. Baltimore, D., for providing lentiviral vector FUGW. We also thank Dr. Elledge, S. J., for providing plasmid pPRIME-CMV-GFP-FF3. We gratefully acknowledge Dr. Rock, K. L., for providing cell line DC2.4 and MF2.2D9. And we thank Dr. Shastri, N., for providing hybridoma T B3Z. This work was supported by National Science Foundation of China (30400392, 30871224, 30801030), National High Technology Research and Development program (863 program) of China (2006AA02Z416), National Key Scientific Research Program (2008ZX10202, 2009ZX09503) and National 973 Project (2007CB512401).

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Authors and Affiliations

  1. Institute of Immunology, PLA, Third Military Medical University, 400038, Chong Qing, China

    Jinyu Zhang, Liyun Zou, Jingyi Li, Jingran Zhou, Na Li, Ting Liu, Ying Wan & Yuzhang Wu

  2. Department of Laboratory Animal Science, Third Military Medical University, 400038, Chong Qing, China

    Qin Liu, Yong Wang & Hong Wei

  3. Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, 58203, USA

    Min Wu

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  1. Jinyu Zhang
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  2. Liyun Zou
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  3. Qin Liu
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  6. Yong Wang
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  7. Na Li
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  9. Hong Wei
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  10. Min Wu
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  11. Ying Wan
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  12. Yuzhang Wu
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Corresponding authors

Correspondence to Ying Wan or Yuzhang Wu.

Additional information

J. Zhang and L. Zou contributed equally to this work.

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Zhang, J., Zou, L., Liu, Q. et al. Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors. Transgenic Res 18, 921–931 (2009). https://doi.org/10.1007/s11248-009-9282-z

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