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Unexpected Rescue of Alpha-synuclein and Multimerin1 Deletion in C57BL/6JOlaHsd Mice by Beta-adducin Knockout

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Abstract

Uniform genetic background of inbred mouse strains is essential in experiments with genetically modified mice. In order to assess Add2 (beta-adducin) function, its null mutation was produced in embryonic stem cells derived from 129Sv mouse and the subsequently obtained mouse mutants were backcrossed for 6 generations with C57BL/6JOlaHsd strain. Comparison of brain proteins between mutated and control animals by two-dimensional gels linked to mass spectroscopy analysis showed expression of Snca (alpha-synuclein) in the mutated animals, but unexpectedly not in the control C57BL/6JOlaHsd mice. Comparison between C57BL/6JOlaHsd and C57BL/6NCrl mice confirmed the presence of a deletion encompassing Snca and in addition Mmrn1 (multimerin1) loci in C57BL/6JOlaHsd strain. The segregation of mutated Add2 together with an adjacent part of the chromosome 6 derived from 129Sv mice, rescued the loss of these two genes in knockout mice on C57BL/6JOlaHsd background. The fact that Add2 knockout was compared with the C57BL/6JOlaHsd mouse strain, which is actually a double knockout of Snca and Mmrn1 emphasizes a need for information provided by commercial suppliers and of exact denominations of substrains used in research.

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Authors and Affiliations

  1. Croatian Institute for Brain Research, School of Medicine, Univeristy of Zagreb, Salata 12, HR-10000, Zagreb, Croatia

    Srećko Gajović, Dinko Mitrečić & Lana Augustinčić

  2. International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34012, Trieste, Italy

    Alessandra Iaconcig & Andrés F. Muro

Authors
  1. Srećko Gajović
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  2. Dinko Mitrečić
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  3. Lana Augustinčić
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  4. Alessandra Iaconcig
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  5. Andrés F. Muro
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Correspondence to Srećko Gajović.

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Gajović, S., Mitrečić, D., Augustinčić, L. et al. Unexpected Rescue of Alpha-synuclein and Multimerin1 Deletion in C57BL/6JOlaHsd Mice by Beta-adducin Knockout. Transgenic Res 15, 255–259 (2006). https://doi.org/10.1007/s11248-006-0003-6

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  • DOI: https://doi.org/10.1007/s11248-006-0003-6

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