Skip to main content

Genetic engineering of peppermint for improved essential oil composition and yield

Abstract

The biochemistry, organization, and regulation of essential oil metabolism in the epidermal oil glands of peppermint have been defined, and most of the genes encoding enzymes of the eight-step pathway to the principal monoterpene component (−)-menthol have been isolated. Using these tools for pathway engineering, two genes and two expression strategies have been employed to create transgenic peppermint plants with improved oil composition and yield. These experiments, along with related studies on other pathway genes, have led to a systematic, stepwise approach for the creation of a ‘super’ peppermint.

This is a preview of subscription content, access via your institution.

References

  • EM Davis KL Ringer ME McConkey R Croteau (2005) ArticleTitleMonoterpene metabolism: cloning, expression and characterization of menthone reductases from peppermint (Mentha × piperita) Plant Physiol 137 873–881 Occurrence Handle10.1104/pp.104.053306 Occurrence Handle15728344

    Article  PubMed  Google Scholar 

  • JM Estévez A Cantero A Reindl S Reichler P León (2001) ArticleTitle1-Deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants J Biol Chem 276 22901–22909 Occurrence Handle10.1074/jbc.M100854200 Occurrence Handle11264287

    Article  PubMed  Google Scholar 

  • J Gershenzon ME McConkey RB Croteau (2000) ArticleTitleRegulation of monoterpene accumulation in leaves of peppermint Plant Physiol 122 205–214 Occurrence Handle10.1104/pp.122.1.205 Occurrence Handle10631264

    Article  PubMed  Google Scholar 

  • BM Lange MR Wildung EJ Stauber C Sanchez D Pouchnik R Croteau (2000) ArticleTitleProbing essential oil biosynthesis and secretion by functional evaluation of expressed sequence tags from mint glandular trichomes Proc Natl Acad Sci USA 97 2934–2939 Occurrence Handle10.1073/pnas.97.6.2934 Occurrence Handle10717007

    Article  PubMed  Google Scholar 

  • X Li Z Gong H Koiwa X Niu J Espartero X Zhu P Veronese B Ruggiero RA Bressan SC Weller PM Hasegawa (2001) ArticleTitleBar-expressing peppermint (Mentha × piperita L. var. Black Mitcham) plants are highly resistant to the glufosinate herbicide Liberty Mol Breed 8 109–118 Occurrence Handle10.1023/A:1013316816955

    Article  Google Scholar 

  • SS Mahmoud RB Croteau (2001) ArticleTitleMetabolic engineering of essential oil yield and composition in mint by altering expression of deoxyxylulose phosphate reductoisomerase and menthofuran synthase Proc Natl Acad Sci USA 98 8915–8920 Occurrence Handle10.1073/pnas.141237298 Occurrence Handle11427737

    Article  PubMed  Google Scholar 

  • SS Mahmoud RB Croteau (2003) ArticleTitleMenthofuran regulates essential oil biosynthesis in peppermint by controlling a downstream monoterpene reductase Proc Natl Acad Sci USA 100 14481–14486 Occurrence Handle10.1073/pnas.2436325100 Occurrence Handle14623962

    Article  PubMed  Google Scholar 

  • SS Mahmoud M Willaims R Croteau (2004) ArticleTitleCosuppression of limonene-3-hydroxylase in peppermint promotes accumulation of limonene in the essential oil Phytochemistry 65 547–554 Occurrence Handle10.1016/j.phytochem.2004.01.005 Occurrence Handle15003417

    Article  PubMed  Google Scholar 

  • D McCaskill R Croteau (1995) ArticleTitleMonoterpene and sesquiterpene biosynthesis in glandular trichomes of peppermint (Mentha × piperita) rely exclusively on plastid-derived isopentenyl diphosphate Planta 197 49–56 Occurrence Handle10.1007/BF00239938

    Article  Google Scholar 

  • D McCaskill J Gershenzon R Croteau (1992) ArticleTitleMorphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.) Planta 187 445–454 Occurrence Handle10.1007/BF00199962

    Article  Google Scholar 

  • ME McConkey J Gershenzon R Croteau (2000) ArticleTitleDevelopmental regulation of monoterpene biosynthesis in the glandular trichomes of peppermint (Mentha × piperita L.) Plant Physiol 122 215–223 Occurrence Handle10.1104/pp.122.1.215 Occurrence Handle10631265

    Article  PubMed  Google Scholar 

  • Murray MJ (1969) Successful use of irradiation breeding to obtain Verticillium-resistant strains of peppermint, Mentha piperita L. In: Induced Mutations in Plants, Proceedings of the International Atomic Energy Agency, Vienna (pp. 345–371).

  • Murray MJ (1971) Additional observations on mutation breeding to obtain Verticillium-resistant strains of peppermint. In: Mutation Breeding for Disease Resistance, Proceedings of the International Atomic Energy Agency, Vienna (pp. 171–195).

  • X Niu X Li P Veronese RA Bressan SC Weller PM Hasegawa (2000) ArticleTitleFactors affecting Agrobacterium tumefaciens-mediated transformation of peppermint Plant Cell Rep 19 304–310 Occurrence Handle10.1007/s002990050017

    Article  Google Scholar 

  • X Niu K Lin PM Hasegawa RA Bressan SC Weller (1998) ArticleTitleTransgenic peppermint (Mentha × piperita L.) plants obtained by co-cultivation with Agrobacterium tumefaciens Plant Cell Rep 17 165–171 Occurrence Handle10.1007/s002990050372

    Article  Google Scholar 

  • KL Ringer EM Davis R Croteau (2005) ArticleTitleMonoterpene metabolism: cloning, expression and characterization of (−)-isopiperitenol/(−)-carveol dehydrogenase from peppermint and spearmint Plant Physiol 137 863–872 Occurrence Handle10.1104/pp.104.053298 Occurrence Handle15734920

    Article  PubMed  Google Scholar 

  • KL Ringer ME McConkey EM Davis GW Rushing R Croteau (2003) ArticleTitleMonoterpene double-bond reductases of the (−)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (−)-isopiperitenone reductase and (+)-pulegone reductase of peppermint Arch Biochem Biophys 418 80–92 Occurrence Handle10.1016/S0003-9861(03)00390-4 Occurrence Handle13679086

    Article  PubMed  Google Scholar 

  • GW Turner R Croteau (2004) ArticleTitleOrganization of monoterpene biosynthesis in Mentha: immunocytochemical localization of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase Plant Physiol 136 4215–4227 Occurrence Handle10.1104/pp.104.050229 Occurrence Handle15542490

    Article  PubMed  Google Scholar 

  • GW Turner J Gershenzon RB Croteau (2000) ArticleTitleDevelopment of peltate glandular trichomes of peppermint (Mentha × piperita L.) Plant Physiol 124 665–679 Occurrence Handle10.1104/pp.124.2.665 Occurrence Handle11027716

    Article  PubMed  Google Scholar 

  • ML Wise R Croteau (1999) Monoterpene biosynthesis DE Cane (Eds) Comprehensive Natural Products Chemistry: Isoprenoids Including Carotenoids and Steroids Elsevier Science Oxford 97–153

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Rodney B. Croteau.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wildung, M.R., Croteau, R.B. Genetic engineering of peppermint for improved essential oil composition and yield. Transgenic Res 14, 365–372 (2005). https://doi.org/10.1007/s11248-005-5475-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11248-005-5475-2

Keywords

  • essential oil
  • Mentha piperita
  • menthofuran
  • menthol
  • monoterpene
  • oil glands
  • peppermint