Transgenic Research

, Volume 14, Issue 5, pp 615–618 | Cite as

Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene

  • Elena Zubko
  • Ivana Macháčková
  • Jiri Malbeck
  • Peter MeyerEmail author
Short communication


The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs.


cytokinins ipt petunia potato Sho gene 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Elena Zubko
    • 1
  • Ivana Macháčková
    • 2
  • Jiri Malbeck
    • 2
  • Peter Meyer
    • 1
    Email author
  1. 1.Centre for Plant SciencesThe University of LeedsUK
  2. 2.Institute of Experimental BotanyPrahaCzech Republic

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