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Transgenic Research

, Volume 13, Issue 6, pp 551–557 | Cite as

Fish eggs as bioreactors: the production of bioactive luteinizing hormone in transgenic trout embryos

  • Tetsuro Morita
  • Goro Yoshizaki
  • Makito Kobayashi
  • Shugo Watabe
  • Toshio Takeuchi
Article

Abstract

We demonstrated the production of goldfish luteinizing hormone (gfLH) by the use of 4-day-old rainbow trout embryos as novel bioreactors. This expression system has several advantages target proteins can be rapidly expressed at low cost, and recombinant proteins can be synthesized at low temperatures and can undergo complex post-translational modifications (PTMs). An expression vector containing gfLH cDNA was microinjected into fertilized trout eggs. After 4 days of incubation at 10°C, transgenic embryos were harvested and glycosylated recombinant gfLH was recovered, which stimulated testosterone production in testicular fragments from the goldfish. This is the first report on the successful production of bioactive recombinant gonadotropin originated from cyprinid. Further, these results demonstrate that trout-embryo bioreactors are a potentially powerful tool for the production of functional recombinant proteins.

Keywords

bioreactor gonadotropin luteinizing hormone rainbow trout recombinant protein transgenic 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Tetsuro Morita
    • 1
  • Goro Yoshizaki
    • 1
  • Makito Kobayashi
    • 2
    • 3
  • Shugo Watabe
    • 3
  • Toshio Takeuchi
    • 1
  1. 1.Department of Marine BiosciencesTokyo University of Marine Science and TechnologyMinato-kuJapan
  2. 2.Department of BiologyInternational Christian UniversityMitakaJapan
  3. 3.Department of Aquatic Bioscience, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan

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