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Hydrophobic Formic Acid Esters for Cofactor Regeneration in Aqueous/Organic Two-Liquid Phase Systems

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Abstract

Hydrophobic formic acid esters have been established as alternative cosubstrates for the formate dehydrogenase (FDH)-catalyzed regeneration of reduced nicotinamides. With this approach challenges related to the ionic nature of the commonly used formate salts, particularly their exclusive water-solubility, can be overcome. Octyl formate was demonstrated to serve as organic phase solubilizing hydrophobic reagents as well as serving as a source of reducing equivalents to enable FDH-catalyzed regeneration of NADH. This system was used to drive a monooxygenase-catalyzed hydroxylation reaction. Phase transfer limitations appear to be the overall rate-limitation of the biphasic reaction system.

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Acknowledgments

This Project was supported by the BIOTRAINS Marie Curie Initial Training Network, financed by the European Union through the 7th Framework People Programme (Grant agreement number 238531).

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Authors and Affiliations

  1. Department of Biotechnology, Delft University of Technology, Julianalaan 136, 2628 BL, Delft, The Netherlands

    Ekaterina Churakova, Isabel Arends & Frank Hollmann

  2. Laboratory of Chemical Biotechnology, Department of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Strasse 66, 44221, Dortmund, Germany

    Bartłomiej Tomaszewski, Katja Buehler & Andreas Schmid

Authors
  1. Ekaterina Churakova
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  2. Bartłomiej Tomaszewski
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  3. Katja Buehler
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  4. Andreas Schmid
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  5. Isabel Arends
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  6. Frank Hollmann
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Corresponding author

Correspondence to Frank Hollmann.

Additional information

Ekaterina Churakova and Bartłomiej Tomaszewski contributed equally.

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Churakova, E., Tomaszewski, B., Buehler, K. et al. Hydrophobic Formic Acid Esters for Cofactor Regeneration in Aqueous/Organic Two-Liquid Phase Systems. Top Catal 57, 385–391 (2014). https://doi.org/10.1007/s11244-013-0195-y

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