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Topics in Catalysis

, Volume 57, Issue 5, pp 339–348 | Cite as

Cryostructured and Crosslinked Viable Cells Forming Monoliths Suitable for Bioreactor Applications

  • Oksana Zaushitsyna
  • Dmitriy Berillo
  • Harald Kirsebom
  • Bo MattiassonEmail author
Original Paper

Abstract

Applications of immobilized biocatalysts in both research and industry require highly active catalysts, preferably at a low cost. In this study, cryogels with high catalyst density were produced through cryostructuration of whole Escherichia coli (E.coli) cells. Prepared cryogels are macroporous materials composed of metabolically active cells crosslinked to each other via polymeric structures. Different macromolecular reagents: oxidized dextran (OxDex), polyvinyl alcohol (PVA) and polyethyleneimine (PEI), the two latter activated with glutaraldehyde (GTA) have been synthesized. Prepared polymers were tested as effective and mild crosslinkers for cells during the cryostructuration procedure. Combination of the two synthetic polymers: PEI+GTA and PVA+GTA was found most suitable for formation of macroporous and stable structures from cells without any toxic effect on them. About 90 % of β-glucosidase activity in cells was retained after crosslinking with a combination of synthetic polymers, whereas E. coli crosslinked using GTA showed complete loss of activity. Preserved viability of cells in cryogel offers possibility to induce protein expression in cells after crosslinking. For β-glucosidase induction post immobilization yielded 50 % activity of that from cells induced in free form before cryo-structurization. The results of the post immobilization studies indicate an interesting potential for handling very sensitive enzymes.

Keywords

Crosslinked cells Cryogel Macromolecular crosslinkers Cell immobilization Enzyme activity 

Notes

Acknowledgments

To funding of Marie Curie Networks for Initial Training fellowship in the BIOTRAINS project (FP7-PEOPLE-ITN-2008-238531) and the financial support from Ångpanneföreningens Foundation for Research and Development is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Oksana Zaushitsyna
    • 1
  • Dmitriy Berillo
    • 1
  • Harald Kirsebom
    • 1
  • Bo Mattiasson
    • 1
    Email author
  1. 1.Department of Biotechnology, Center for Chemistry and Chemical EngineeringLund UniversityLundSweden

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