Abstract
Two zinc(II) complexes with S-alkenyl derivatives of thiosalicylic acid as ligands have been synthesized and characterized by microanalysis, IR, 1H and 13C NMR spectroscopy. The complexes were obtained by direct reaction of ZnCl2 with S-alkenyl derivatives of thiosalicylic acid in aqueous solution. On the basis of the physico-chemical and spectroscopic data, we conclude that the ligands are bidentately coordinated to the zinc(II) center. The interaction of the complexes with calf thymus DNA (CT-DNA) has been investigated by absorption (UV–Vis) and ethidium bromide displacement studies. The cytotoxic activities of the complexes were evaluated in comparison with oxaliplatin and cisplatin against two different cancer cell lines: murine colon carcinoma (CT26) and mouse melanoma (B16F1), while non-cancerous mouse mesenchymal stem cells (mMSCs) were used as a control. Both complexes showed moderate activities against mouse colon cancer and melanoma cells. The decrease in viability of melanoma cells is caused by induction of apoptosis and G2 phase arrest. The zinc(II) complex with S-propenyl thiosalicylic acid, although has a significantly weaker cytotoxic effect on tumor cells than oxaliplatin and cisplatin, acts more selectively on tumor cells than the known drugs.
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References
Strohfeldt KA (2015) Essentials of inorganic chemistry: for students of pharmacy, pharmaceutical sciences and medicinal chemistry. Wiley, University of Reading
Wegmüller R, Tay F, Zeder C, Brnić M, Hurrell RF (2013) Zinc absorption by young adults from supplemental zinc citrate is comparable with that from zinc gluconate and higher than from zinc oxide. J Nutr 144:132–136
McCall KA, Huang CC, Fierke CA (2000) Function and mechanism of zinc metalloenzymes. J Nutr 130:1437–1446
Prasad AS (2003) Zinc deficiency: has been known of for 40 years but ignored by global health organisations. BMJ 326:409–410
Lansdown AB, Mirastschijski U, Stubbs N, Scanlon E, Ågren MS (2007) Zinc in wound healing: theoretical, experimental, and clinical aspects. Wound Repair Regen 15:2–16
Hoogenraad TU (2006) Paradigm shift in treatment of Wilson’s disease: zinc therapy now treatment of choice. Brain Dev 28:141–146
Dunn MF (2005) Zinc-ligand interactions modulate assembly and stability of the insulin hexamer—a review. Biometals 18:295–303
Tuerk MJ, Fazel N (2009) Zinc deficiency. Curr Opin Gastroenterol 25:136–143
Meunier N, Feillet-Coudray C, Rambeau M, Andriollo-Sanchez M, Brandolini-Bunlon M, Coulter SJ, Cashman KD, Mazur A, Coudray C (2005) Impact of micronutrient dietary intake and status on intestinal zinc absorption in late middle-aged men: the ZENITH study. Eur J Clin Nutr 59:S48
Federico A, Iodice P, Federico P, Del Rio A, Mellone MC, Catalano G, Federico P (2001) Effects of selenium and zinc supplementation on nutritional status in patients with cancer of digestive tract. Eur J Clin Nutr 55:293–297
Prasad AS, Beck FW, Doerr TD, Shamsa FH, Penny HS, Marks SC, Kaplan J, Kucuk O, Mathog RH (1998) Nutritional and zinc status of head and neck cancer patients: an interpretive review. J Am Coll Nutr 17:409–418
Gauna GA, Marino J, Vior MC, Roguin LP, Awruch J (2011) Synthesis and comparative photodynamic properties of two isosteric alkyl substituted zinc(II) phthalocyanines. Eur J Med Chem 46:5532–5539
Enyedy ÉA, Nagy NV, Zsigó É, Kowol CR, Arion VB, Keppler BK, Kiss T (2010) Comparative solution equilibrium study of the interactions of copper(II), iron(II) and zinc(II) with triapine(3-aminopyridine-2-carbaldehyde thiosemicarbazone) and related ligands. Eur J Inorg Chem 2010:1717–1728
Li MX, Zhang LZ, Yang M, Niu JY, Zhou J (2012) Synthesis, crystal structures, in vitro biological evaluation of zinc(II) and bismuth(III) complexes of 2-acetylpyrazine N(4)-phenylthiosemicarbazone. Bioorg Med Chem Lett 22:2418–2423
Singh AK, Pandey OP, Sengupta SK (2012) Synthesis, spectral characterization and biological activity of zinc(II) complexes with 3-substituted phenyl-4-amino-5-hydrazino-1,2,4-triazole Schiff bases. Spectrochim Acta A Mol Biomol Spectrosc 85:1–6
Abdel-Mawgoud AM, Abdel-Hamid R (1987) Cobalt(II), copper(II), zinc(II)-amino and thiosalicylic acids ternary complexes. Monatsh Chem 118:1219–1223
Nikolić MV, Mijajlović MŽ, Tomović DLJ, Bukonjić AM, Jevtić VV, Ratković ZR, Trifunović SR, Radić GP (2018) Synthesis and characterization of zinc(II)-complexes with S-alkyl derivatives of thiosalicylic acid. Serb J Exp Clin Res 19:113–117
Nikolić MV, Mijajlović MŽ, Jevtić VV, Ratković ZR, Novaković SB, Bogdanović GA, Milovanović J, Arsenijević A, Stojanović B, Trifunović SR, Radić GP (2016) Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid. J Mol Struct 1116:264–271
Tomovic DLj, Bukonjic AM, Kocovic A, Nikolic MV, Mijajlovic MZ, Jevtic VV, Ratkovic ZR, Arsenijevic AN, Milovanovic JZ, Stojanovic B, Trifunovic SR (2017) Synthesis, characterization, and cytotoxicity of binuclear cooper (II)-complexes with some S-alkenyl derivatives of thiosalicyclic acid. Serb J Exp Clin Res 18:13–18
Dimiza F, Fountoulaki S, Papadopoulos AN, Kontogiorgis CA, Tangoulis V, Raptopoulou CP, Psycharis V, Terzis A, Kessissoglou DP, Psomas G (2011) Non-steroidal antiinflammatory drug-copper(II) complexes: structure and biological perspectives. Dalton Trans 40:8555–8568
Dimiza F, Perdih F, Tangoulis V, Turel I, Kessissoglou DP, Psomas G (2011) Interaction of copper(II) with the non-steroidal anti-inflammatory drugs naproxen and diclofenac: synthesis, structure, DNA-and albumin-binding. J Inorg Biochem 105:476–489
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
Ojeda JJ, Romero-Gonzalez ME, Pouran HM, Banwart SA (2008) In situ monitoring of the biofilm formation of Pseudomonas putida on hematite using flow-cell ATR-FTIR spectroscopy to investigate the formation of inner-sphere bonds between the bacteria and the mineral. Min Mag 72:101–106
Gao X, Metge DW, Ray C, Harvey RW, Chorover J (2009) Surface complexation of carboxylate adheres Cryptosporidium parvum oocysts to the hematite-water interface. Environ Sci Technol 43:7423–7429
Novakova O, Chen H, Vrana O, Rodger A, Sadler PJ, Brabec V (2003) DNA interactions of monofunctional organometallic ruthenium(II) antitumor complexes in cell-free media. Biochemistry 42:11544–11554
Recio Despaigne AA, Da Silva JG, da Costa PR, dos Santos RG, Beraldo H (2014) ROS-mediated cytotoxic effect of copper(II) hydrazone complexes against human glioma cells. Molecules 19:17202–17220
Silconi ZB, Benazic S, Milovanovic J, Jurisevic M, Djordjevic D, Nikolic M, Mijajlovic M, Ratkovic Z, Radić G, Radisavljevic S, Petrovic B, Radosavljevic G, Milovanovic M, Arsenijevic N (2018) DNA binding and antitumor activities of platinum(IV) and zinc(II) complexes with some S-alkyl derivatives of thiosalicylic acid. Transit Met Chem. https://doi.org/10.1007/s11243-018-0260-2
Dhar S, Nethaji M, Chakravarty AR (2005) Effect of charge transfer bands on the photo-induced DNA cleavage activity of [1-(2-thiazolylazo)-2-naphtholato] copper(II) complexes. J Inorg Biochem 99:805–812
Čanović P, Simović AR, Radisavljević S, Bratsos I, Demitri N, Mitrović M, Zelen I, Bugarčić ŽD (2017) Impact of aromaticity on anticancer activity of polypyridyl ruthenium (II) complexes: synthesis, structure, DNA/protein binding, lipophilicity and anticancer activity. J Biol Inorg Chem 22:1007–1028
Velma V, Dasari SR, Tchounwou PB (2016) Low doses of cisplatin induce gene alterations, cell cycle arrest, and apoptosis in human promyelocytic leukemia cells. Biomark Insights 11:113–121
Sarin N, Engel F, Kalayda GV, Mannewitz M, Cinatl J Jr, Rothweiler F, Michaelis M, Saafan H, Ritter CA, Jaehde U, Frötschl R (2017) Cisplatin resistance in non-small cell lung cancer cells is associated with an abrogation of cisplatin-induced G2/M cell cycle arrest. PLoS ONE 12:e0181081
Lützkendorf J, Wieduwild E, Nerger K, Lambrecht N, Schmoll HJ, Müller-Tidow C, Müller LP (2017) Resistance for genotoxic damage in mesenchymal stromal cells is increased by hypoxia but not generally dependent on p53-regulated cell cycle arrest. PLoS ONE 12:e0169921
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This work was funded by grants from the Serbian Ministry of Science and Technological Development (Grant Nos. ON175069, ON175071, ON172016, ON172034 and ON172011).
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Popović, A., Nikolić, M., Mijajlović, M. et al. DNA binding and antitumor activities of zinc(II) complexes with some S-alkenyl derivatives of thiosalicylic acid. Transit Met Chem 44, 219–228 (2019). https://doi.org/10.1007/s11243-018-0285-6
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DOI: https://doi.org/10.1007/s11243-018-0285-6