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Synthesis, characterization, DNA binding and nuclease activity of binuclear copper(II) complexes of cuminaldehyde thiosemicarbazones

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Abstract

Binuclear copper(II) complexes of thiosemicarbazones derived from cuminaldehyde (p-isopropyl benzaldehyde) and substituted thiosemicarbazides NH2NHC(S)NHR, where R = H, Me, Et or Ph have been synthesized and characterized. The ESR indicates that the dissociation of dimeric complex into mononuclear [Cu(L)Cl(DMSO)3] units in polar solvents like DMSO, where L = monoanionic thiosemicarbazone. The molecular ion peak in the LC-MS coincides with the formula weight of the complexes. The absorption titration studies revealed that each of these complexes is an avid binder to calf thymus-DNA. The apparent binding constants are in the order of 107–108 M−1. The nucleolytic cleavage activities of the ligands and their complexes were assayed on pUC18 plasmid DNA using gel electrophoresis in the presence and absence of H2O2. The ligands showed increased nuclease activity when administered as copper complexes. All these copper(II) complexes behave as an efficient chemical nucleases with hydrogen peroxide activation. These studies revealed that the complexes exhibit both oxidative and hydrolytic chemistry in DNA cleavage.

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Acknowledgements

The authors thank to RSIC, Indian Institute of Technology-Bombay, Mumbai and CDRI, Lucknow for providing ESR and Mass spectral data, respectively.

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Authors and Affiliations

  1. Department of Chemistry, Sri Krishnadevaraya University, Anantapur, 515 003, AP, India

    Panchangam Murali Krishna & Katreddi Hussain Reddy

  2. Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, AP, India

    Jay Prakash Pandey & Dayananda Siddavattam

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  1. Panchangam Murali Krishna
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  2. Katreddi Hussain Reddy
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  3. Jay Prakash Pandey
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  4. Dayananda Siddavattam
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Correspondence to Katreddi Hussain Reddy.

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Murali Krishna, P., Hussain Reddy, K., Pandey, J.P. et al. Synthesis, characterization, DNA binding and nuclease activity of binuclear copper(II) complexes of cuminaldehyde thiosemicarbazones. Transition Met Chem 33, 661–668 (2008). https://doi.org/10.1007/s11243-008-9094-7

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