Abstract
The interaction of DNA with Copper(II)-Glutathione (CuGSH) has been investigated by various biophysical methods. The interaction ratio of DNA and Copper(II)-Glutathione in solution phase has been determined spectrophotometrically and found to be 0.25. EPR spectroscopy and UV–Vis findings suggest that Cu(II)-Glutathione neither bound to the DNA bases covalently nor intercalated, this has further been substantiated by the determination of intrinsic binding constant (2.1 × 102). Viscometric measurements also support this type of binding to DNA by Cu(II)-Glutathione. EPR studies and visible d–d spectra of CuGSH after interaction with DNA, suggested that Copper remained in the Copper(II) state. DNA conformations after interaction with Cu(II)-Glutathione has been determined spectroscopically. Circular dichroism studies revealed that the B conformation of DNA is changed to A after interaction with Cu(II)-Glutathione. This has further been substantiated by thin film IR (Infrared) studies.
Similar content being viewed by others
References
Wang AHJ, Quigley GJ, Kolpak FJ (1981) Science 211:171
Wang AHJ, Quigley GJ, Kolpak FJ, Grawford JL, Van Boom JH, Mareland GV, Rich A (1979) Nature 282:680
Berger I, Winston W, Manoharan R, Schwartz T, Alfken J, Kim YG, Lowenhaupt K, Herbert A, Rich A (1998) Biochemistry 37:13313
Neault JF, Tajmir-Riahi HA (1998) J Phys Chem B 102:1610
Mefail-Isom L, Shuiand X, Williams LD (1998) Biochemistry 37:17105
Miduturu CV, Silverman SK (2006) Angew Chem Ed 45:1918
Murphy CJ, Arkin MR, Jenkis Y, Ghatlia ND, Bossman-Turro NJ, Barton JK (1993) Science 262:1025
Yan CX, Wei YB, Yang P (2006) Chinese J Chem 24:1006
Zhao Y, Zhu J, He W, Yang Z, Zhu Y, Li Y, Zhang J, Guo Z (2006) Chemistry Euro J 12:6621
Arkin MR, Stemp EDA, Holmlin RE, Barton JK, Hormann A, Olson EJC, Barbara PF (1996) Science 273:475
Erkkila KE, Odom DT, Barton JK (1999) Chem Rev 99:2777
Shen D, Dalton TP, Nebert DW, Shertzer HG (2005) J Biol Chem 280:25305
Arrigo AP (1999) Free Radic Biol Med 27:936
Sen CK, Sies H, Baeuerle PA (1999) Antioxidant and redox regulation of genes. Academic press, San Diego
Tormos C, Chaves F, Garcia MF, Garrido F, Jover R, O’Connor JE, Oliva A, Saez GT (2004) Cancer Lett 208:103
Martin MB, Reiter R, Pham T, Avetecost YR, Pratap K, Gilmore BA, Divekar S, Dagata RS, Bull JL, Stoica A (2003) Endocrinology 144:2425
Ivanov AV, Korneeva EV, Gerasimenko AV, Forsling W (2005) Russ J Coord Chem 31:695
Hu S, Furst P, Hamer D (1990) New Biol 2:544
Prutz WA (1994) Biochem J 302:373
Mukherjea KK, Panda G (2004) J Indian Chem Soc 81:210
Chaires JB, Dattagupta N, Crothers DM (1982) Biochemistry 21:933
Muller W, Crothers DM (1968) J Mol Biol 35:252
Aboukaïs A, Abi-Aad E, Bennani A, Chachaty C, Bonnelle JP (1995) J Chem Soc Faraday Trans 91:3299
Satyanarayana S, Dabrowiak JC, Chaires JB (1992) Biochemistry 31:9319
Tinoco I, Bustamante JC, Maestre MF (1980) Annu Rev Biophys Bioeng 9:107
Maestre MF (1970) J Mol Biol 52:543
Olmsted J, Kearns DR (1977) Biochemistry 16:3647
Reinert KK (1972) J Mol Biol 72:593
Dirico DE, Keller JPB, Hartman KA (1985) Nucleic Acids Res 13:251
Mukherjea KK, Bhattacharyya RG (1993) J Inorg Biochem 52:27
Reed CJ, Douglas KT (1991) Biochem J 275:601
Acknowledgments
Authors are thankful to the University Grants Commission, New Delhi for financial assistance to K.K.M in the form of a MRP NO F-12-10/98 (SR-I), where GP had been a project fellow. We are also thankful to Jadavpur University for assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mukherjea, K.K., Panda, G. & Selim, M. Biophysical, spectroscopic and biochemical investigation of DNA–Cu(II)-GSH interactions. Transition Met Chem 33, 203–210 (2008). https://doi.org/10.1007/s11243-007-9034-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11243-007-9034-y