Abstract
Lack of a rapid and robust in vitro regeneration protocol for Tossa jute (Corchorus olitorius L.) hinders the genetic transformation of jute crops for different agronomic traits of interest. The current study was conducted to develop a rapid, efficient, and reproducibleregeneration protocol for recalcitrant Tossa jute. The potential of auxins (IAA, IBA) and cytokinin (BAP) for the induction of callus, regeneration of shoot and formation of root from the cotyledon with attached petiole of cultivar O-9897 on MS was tested accordingly. Approximately, total 96 calli (out of 100 explants) were induced within 32 days on MS media harboring 2.5 mg/L BAP + 0.5 mg/L IAA. Within 5 weeks, about 95 calli regenerated total 99 shoots (86.6%) after 8–10 days of culture. Rooting of 52 regenerants (52.5%) was initiated on half-strength MS media supplemented with 1.0 mg/L IBA within 10 days. After acclimatization, a total of 52 rooted plantlets were transferred to the soil pot and 45 (86.53%) plantlets were survived and turned to normal plants without somaclonal variations. It is the first investigation analyzing foliar micro-morphological characters like stomata, trichomes, and vein patterning of leaves from in-vitro grown progeny and acclimatized plants that assess the developmental adaptation of micropropagated plantlets for survival under field conditions. The findings demonstrate morphological homogeneity between micropropagated plants regenerated from cotyledon explants with attached petioles and the mother plants. This is the pioneering report on foliar micromorphological analysis at subsequent micropropagation stages, confirming the phenotypic fidelity of regenerants.
Key message
In vitro regeneration of Corchorus olitorius L. is quite difficult and even nearly impossible that hinders the genetic transformation of jute crops. The current research wills unravel this difficult job into possibility.
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Data availability
The data that support the findings of this study are available upon request from the corresponding author.
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Acknowledgements
This research is an integrated part of Basic and Applied Research on Jute (BARJ) project hosted by Bangladesh Jute Research Institute (BJRI) and funded by the Ministry of Agriculture, The People’s Republic of Bangladesh. We highly acknowledge the Ministry of Agriculture for the financial support throughout the research. No financial support was received during manuscript preparation. Authors are highly grateful to Mr Md. Ruhul Amin, Senior Scientific Officer of BJRI for his earnest support throughout the experiment.
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Borhan Ahmed and Md Jakir Hossain were planned and designed this current research. Md Jakir Hossain and Rebina Ferdous conducted all laboratory research, data collection and analysis. Data analysis and the preparation of microscopic images were performed by Afsana Mimi. Borhan Ahmed, Afsana Mimi, and Quazi Mosaddeque Hosen revised and improved the manuscript. Overall research activities were conducted under the supervision of Borhan Ahmed. The primary manuscript was developed by Md Jakir Hossain and all authors read and approved the final manuscript.
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Communicated by Yan Liu.
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Hossain, M.J., Ahmed, B., Mimi, A. et al. Robust and reliable in vitro regeneration of Tossa Jute (Corchorus olitorius L. var. O-9897). Plant Cell Tiss Organ Cult 157, 50 (2024). https://doi.org/10.1007/s11240-024-02772-y
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DOI: https://doi.org/10.1007/s11240-024-02772-y