Abstract
Clonal forestry is an important approach for intensive management as it involves vegetative propagation, which does not entail gene separation and recombination, thereby retaining the excellent traits of the parent trees. Exploring essential genes involved in rooting of cuttings seems urgent in Catalpa bungei as it is the capital method in vegetative propagation. In this study, we identified a homolog of the SHR gene involved in the development of adventitious roots (ARs) in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, and the expression of the CbSHR gene reaches its peak at the callus differentiation stage (S4, about 30 days after cutting) during AR development. By overexpressing the CbSHR gene in tobacco, the number of roots and their length were increased compared with the wild-type line, thus identifying a pivotal homolog, the CbSHR gene in C. bungei, which promotes the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei.
Key Message
In this study, we identified a homolog of CbSHR gene in C. bungei through multiple alignment, homologous cloning, qPCR detection, and transgenic techniques. The CbSHR gene encodes a protein of 445 amino acids, the expression of the CbSHR gene reaches its peak at the callus differentiation stage during branches cutting. Heterologous overexpression of CbSHR gene in tobacco displays a booming ARs, including RN (number of roots) and RL (root length) when compared with the wild-type line. Thus, we identified a pivotal homolog of CbSHR gene in C. bungei, which promoted the initiation and elongation of ARs. This result provided a candidate gene for genetic improvement of cutting, and will also contribute to understanding the molecular mechanisms of AR development in C. bungei.
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Acknowledgements
The authors are appreciated about the platform supported by the Forestry Biotechnology Laboratory of Henan University of Science and Technology.
Funding
This study was financially funded by the PhD Research Startup Foundation of Henan University of Science and Technology (grant no. 13480076 to Z.Y.H.), College Students' Innovative Entrepreneurial Training Plan Program of Henan University of Science and Technology (grant no. 2023451 to J.Y.L.) and the National Natural Science Foundation of China (grant no. 32171701).
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Jiawen Yang and Ziyuan Hao conceived the study, Jingyi Liu and Ziyuan Hao participated in the entire experimental process as well as statistical analyses, total RNA and the full-length cDNA isolation was performed by Jingyi Liu and Xinchang Wang, plant culture of the tobaccos were contributed by Jingyi Liu, Yao Teng, and Peiyao Jiang, quantitative real-time PCR of different tissues were provided by Jingyi Liu, Fuqiang Yang and Ziyuan Hao. Jingyi Liu and Hongying Li completed the protein properties analysis. Jingyi Liu and Weijie Si participated in root length determination and data statistics. Ziyuan Hao and Jingyi Liu drafted the manuscript and the further revision was completed by Ziyuan Hao and Jiawen Yang. Jiawen Yang and Ziyuan Hao is responsible for the manuscript as a whole.
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Hao, Z., Liu, J., Teng, Y. et al. Identification and functional analysis of a CbSHR homolog in controlling adventitious root development in Catalpa bungei. Plant Cell Tiss Organ Cult 157, 7 (2024). https://doi.org/10.1007/s11240-024-02730-8
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DOI: https://doi.org/10.1007/s11240-024-02730-8