Abstract
The utilization of temporary immersion system-bioreactors (TIB) has shown promise for sugarcane micropropagation due to its capacity for high-yield seedlings production. However, the temporary immersion system also carries the risk of somaclonal variations (SV) in regenerated plants. SV, encompassing phenotypic changes resulting from genetic or epigenetic alterations, and it presents challenges in preserving the genetic uniformity of the seedlings, a crucial factor for sugarcane productivity. A molecular marker like Simple Sequence Repeats (SSR) offers a reliable means to assess the genetic stability of TIB-derived sugarcane seedlings, ensurinng that the temporary immersion system-based micropropagation produces true-to-type planting materials. In this study, SSR analysis was conducted to determine the relative genetic stability of TIB-derived sugarcanes, using 15 SSR markers, generating over 200 DNA fragments ranging from 150 to 4,000 bp in size. The genetic stability was calculated as the Jaccard’s similarity index of the SSR patterns of TIB-derived sugarcanes compared to their mother plants. The genetic stability varied between 92 and 96% in three Indonesian sugarcane varieties (PSKA 942, PS 094, and PS 091) which is relatively high considering the high cell mass multiplication in TIB (up to 30 multiplies). Morphological observations in the field also revealed no differences between TIB-derived sugarcane and their mother plants, convincing the true-to-type of micropropagation products. This study demonstrated the high relative genetic stability of TIB-derived sugarcanes, paving the way for large-scale commercial application in plantations.
Key message
Genetic stability test using SSR markers revealed that a high yield micropropagation of sugarcane using TIB produced true-to-type sugarcane seedlings, also confirmed by the morphological uniformity on the field observations.
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Data Availability
The datasets generated or analyzed during the current study are available from the corresponding author upon reasonable request.
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Acknowledgements
This work is part of the project “Automatization and scale up production of the superior sugarcane clonal seedlings in the Temporary Immersion Bioreactor”, funded by the Indonesian Endowment Fund for Education (LPDP-RI) through the grant research of RISPRO Invitasi 2020.
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RTS conducted experiments and carried out data analysis on the sugarcane micropropagation, prepared graphs, figures, and manuscript; AAA conducted experiments and carried out data analysis on the genetic stability assessment, and prepared the manuscript; YS conducted experiments on the DNA preparation and prepared the manuscript; IR supervised the sugarcane TIB culture; HM conceptualized and supervised the genetic stability assessment; SL provided and prepared the sugarcane plant materials for all experiments, and conducted field observation; MERBP conducted micropropagation initiation and acclimatization; MMS conducted histological analysis; SS conceptualized the research design on the sugarcane micropropagation, evaluated and edited the manuscript.
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Communicated by Jericó Jabín Bello-Bello.
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Saptari, R.T., Aksa, A.A., Riyadi, I. et al. Genetic stability analysis of the temporary immersion bioreactors–derived sugarcane seedlings with simple sequence repeat (SSR) markers. Plant Cell Tiss Organ Cult 156, 22 (2024). https://doi.org/10.1007/s11240-023-02657-6
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DOI: https://doi.org/10.1007/s11240-023-02657-6