Abstract
Scutellaria baicalensis Georgi. is a valuable medicinal plant. The hairy roots strain of S. baicalensis (Sc. baic.-1) from IPP RAS collection was used as a model system for the study. Effects of 1 and 10 mM of hydrogen peroxide (H2O2) were first reported on the growth characteristics of hairy roots, fatty acid (FAs) composition of total lipids, and major root-specific flavones during the cultivation cycle. H2O2 at a concentration of 1 mM exhibited the properties of a signaling molecule: the total FAs content of total lipids increased mainly due to polyunsaturated FAs, including very-long-chain FAs (VLCFAs); the total flavones content was about 1.5-fold higher than in the control. Besides, at 1 mM H2O2 the content of aglycones, especially wogonin, was higher than in the other variants. At 10 mM H2O2, the properties of H2O2 as reactive oxygen species were manifested: inhibition in the growth of hairy root culture was observed against the background of an increase in the content of malondialdehyde; the proportion of saturated FAs and the total proportion of VLCFAs increased; the amount of the aglycone baicalein was also significantly reduced. Based on the data obtained, we can speak about a possible role of VLCFAs in the protection of S. baicalensis roots against oxidative stress.
Key message
Hydrogen peroxide (H2O2) has a dose-dependent effect on the hairy roots of Scutellaria baicalensis, 1 mM H2O2 stimulates the synthesis of flavones, 10 mM H2O2 increases the absolute amount of fatty acids of lipids, but in both cases increases the content of very-long-chain fatty acids.
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The research was carried out within the state assignment of Ministry of Science and Higher Education of the Russian Federation (theme No. 121050500047-5 and 122042700043-9).
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AIS and AYS developed the concept, designed the experiments, and wrote the manuscript. TVI and ASV performed the majority of the experimental work, analyzed the data, and revised the manuscript. YMP carried out the HPLC analysis. All authors read and approved of its content.
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Solov’eva, A.I., Stepanova, A.Y., Ivanova, T.V. et al. Dose-dependent effect of H2O2 on hairy roots of Scutellaria baicalensis: growth, composition of fatty acids, and flavones. Plant Cell Tiss Organ Cult 155, 893–905 (2023). https://doi.org/10.1007/s11240-023-02609-0
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DOI: https://doi.org/10.1007/s11240-023-02609-0