Abstract
Through artificial polyploid induction, new varieties of horticultural crops with better quality can be generated. Mixed culture was used to induce polyploidy of black currant in this study. Sterile seeds of black currant were treated with different concentrations of colchicine (0.05%, 0.10%, 0.15%, 0.20%) for different lengths of time (8 h, 12 h, 24 h, 36 h, 48 h). The results showed that 0.15% colchicine treatment for 24 h was the best condition for polyploid induction of black currant, and the highest induction rate was 12.0%. The ploidy of seedlings was verified by chromosome counting and flow cytometry. The morphological differences and stomatal characteristics of tetraploid plants and diploid plants were compared. The tetraploid plants were characterized by being dwarfed with shorter internode lengths, having thick stems,t larger and rounder leaves, with decreased stomatal density and larger stomates. Finally, a total of 3246 differentially expressed genes were identified in diploid and tetraploid leaves by transcriptome sequencing analysis, including 615up and 2631down regulated genes. KEGG (Kyoto Encyclopedia of Genes and Genome) pathway analysis showed that 1355 DEGs (Differentially expressed gene) were enriched in 133 metabolic pathways. DEGs were mainly enriched in ‘Plant hormone signal transduction’ and ‘MAPK (Mitogen-activated protein kinase) signaling pathway-plant’ pathways, that were related to plant hormones, biotic stress and abiotic stress signal transduction. This study enriched the germplasm resources of black currant, provided a new way for black currant breeding, and provided a theoretical basis for revealing the potential mechanism of tetraploid development.
Key message
Successfully induced tetraploid blackcurrant, revealing the difference between tet raploid and diploid blackcurrant plants through physiological characteristics and transcriptome analysis.
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Data availability
Additional data are included in the supplementary information file. Furthermore, all the datasets generated during the current study are available from the corresponding author on request.
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Acknowledgements
Gansu Provincial Science and Technology Plan Project (22CX8NE193); Tianjin Science and Technology Plan Project (16PTZSTG00020); Tianjin Jinnan District National Agricultural Science and Technology Park ‘one core three areas’ special project (20YHXSQ005); Tianjin Binhai New Area Science and Technology Project (BHXQKJXM-SF-2018-33).
Funding
This study was funded by Gansu Provincial Science and Technology Plan Project (Grant Number 22CX8NE193), Tianjin Science and Technology Plan Project (Grant Number 16PTZSTG00020), Tianjin Jinnan District National Agricultural Science and Technology Park ‘one core three areas’ special project (Grant Number 20YHXSQ005), Tianjin Binhai New Area Science and Technology Project (Grant Number BHXQKJXM-SF-2018-33).
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RD was responsible for tetraploid induction and transcriptome analysis. YP and JN designed and guided the experiment. LH and HY were responsible for sorting and analyzing the data. YC and YL were responsible for the supervision and audit of the experiment.
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Communicated by Maria Antonietta Germanà.
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Dong, R., Pei, Y., Hong, L. et al. Tetraploid induction identification and transcriptome preliminary analysis of black currant (Ribes rubrum L.). Plant Cell Tiss Organ Cult 155, 861–872 (2023). https://doi.org/10.1007/s11240-023-02605-4
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DOI: https://doi.org/10.1007/s11240-023-02605-4