Abstract
Lilium pumilum plays an important role in resistance breeding of lily because of its versatile value and strong stress resistance. It is of great significance to study the conservation of its resources. To improve its vitality after cryopreservation, the effect of abscisic acid (ABA) on the viability of cryopreserved shoot tips and its mechanism were studied based on the establishment of cryopreservation-vitrification protocol of Lilium pumilum. When 25 μM abscisic acid was added into the preculture medium, the survival rate after cryopreservation was increased by 22.73% and the regeneration rate by 11.48%, reaching 93.64% and 63.33%, respectively. Adding exogenous abscisic acid increased the contents of endogenous osmotic regulatory substances proline, soluble sugar and soluble protein and reduced the activities of caspase-3-like and caspase-9-like enzyme that characterize programmed cell death (PCD). This suggests that the addition of abscisic acid during preculture phase of cryopreservation improved viability of shoot tips by affecting osmotic stress and programmed cell death.
Key message
A simple and efficient cryopreservation procedure for the shoot tips of Lilium pumilum was established. Exogenous abscisic acid increased the viability of shoot tips by regulating osmotic stress and programmed cell death.
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Data Availability
The datasets in the current study are available from the corresponding author on reasonable request.
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This research was supported by the National Natural Science Foundation of China (Nos. 31770741).
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QW designed the research, analyzed the data and drafted the manuscript. MZ completed the experiment. LZ offered some help on material collection. YL conceived the project, supervised the analysis and critically revised the manuscript. All authors read and approved the manuscript.
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Wang, Q., Zhu, M., Zhang, L. et al. Abscisic acid increases the viability of cryopreserved shoot tips of Lilium pumilum by regulating the osmotic stress and programmed cell death. Plant Cell Tiss Organ Cult 155, 731–741 (2023). https://doi.org/10.1007/s11240-023-02594-4
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DOI: https://doi.org/10.1007/s11240-023-02594-4