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Impact of kinetin on essential oil content in Acmella oleracea

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Abstract

The use of growth regulators in in vitro plant cultures is a common practice in agriculture, and has been known to improve plant development and affect chemical composition in plants that produce essential oils. In this study, the researchers investigated the effect of kinetin, a growth regulator, on the chemical composition of Acmella oleracea essential oil produced in vitro. The experiment consisted of four treatments with varying concentrations of kinetin added to the culture medium: T1 (control), T2 (0.1 mg L−1), T3 (0.3 mg L−1), and T4 (0.5 mg L−1). After 90 days of growth in a controlled environment, the leaves were harvested and hydrodistilled to obtain the essential oil. Gas chromatography coupled with mass spectrometry (GC–MS) was used for chemical characterization of the essential oil. The results showed that the concentrations of the major compounds in A. oleracea essential oil were influenced by the addition of kinetin to the culture medium. Specifically, the concentrations of 2-tridecanone and trans-farnesene increased with increasing levels of kinetin, while the concentrations of Germacrene D and 8-cedren-13-ol decreased with increasing levels of kinetin. These findings suggest that the addition of kinetin can influence the chemical composition of A. oleracea essential oil produced in vitro. Further studies are underway to evaluate the effect of other compounds on the chemical composition of A. oleracea essential oil.

Key message

Kinetin addition to in vitro cultures of Acmella oleracea influences the chemical composition of its essential oil, increasing 2-tridecanone and trans-farnesene, while decreasing Germacrene D and 8-cedren-13-ol.

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Data Availability

Data available upon request to the corresponding author.

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Acknowledgements

The authors are grateful to the Postgraduate Program in Biotechnology Applied to Agriculture of Paranaense University for financial support and fellowship.

Funding

This work was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação Araucária.

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Authors and Affiliations

  1. Postgraduate Program in Biotechnology Applied to Agriculture, Biotechnology Department, Paranaense University, Umuarama, Paraná, Brazil

    Nastassja Kimberlly Lima, Eloisa Schneider da Silva, Rayane Monique Sete da Cruz, Julliane Destro de Lima, Cristian Coelho da Silva, Mayara dos Santos Queiroz & Glacy Jaqueline da Silva

  2. Natural Products Chemistry Laboratory, Postgraduate Program in Biotechnology Applied to Agriculture, Paranaense University, Umuarama, Paraná, Brazil

    Zilda Cristiani Gazim

  3. Postgraduate Program in Clean Technologies, Cesumar University—Unicesumar, and Cesumar Institute of Science, Technology and Innovation—ICETI, Maringa, Brazil

    José Eduardo Gonçalves

  4. Faculty of Agronomic Engineering, Universidad Nacional del Este, Minga Guazú, Paraguay

    Daisy Leticia Ramirez Monzon

  5. Faculty of Agronomic Engineering, Paranaense University, Umuarama, Paraná, Brazil

    Renan Rizzato Espessato

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  1. Nastassja Kimberlly Lima
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Contributions

NKL: Conceptualization, Methodology—Original draft preparation; ESS: Methodology; RMSC: Methodology; JDL: Methodology, Data curation, Writing, and Editing; CCS: Writing—Reviewing and Editing; MSQ: Reviewing and Editing; ZCG: Methodology, Resources, Funding acquisition; JEG: Methodology, Resources, Funding acquisition; DLRM: Writing—Reviewing and Editing; RRE: Writing—Reviewing and Editing; GJS: Conceptualization, Visualization, Writing—Reviewing and Editing, Resources, Funding acquisition.

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Correspondence to Glacy Jaqueline da Silva.

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Lima, N.K., da Silva, E.S., da Cruz, R.M.S. et al. Impact of kinetin on essential oil content in Acmella oleracea. Plant Cell Tiss Organ Cult 153, 539–546 (2023). https://doi.org/10.1007/s11240-023-02489-4

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