Abstract
Cattleya warneri, an orchid with ornamental potential, suffers indiscriminate harvest and is classified as a vulnerable species, requiring propagation studies. The aim of this study was to evaluate the effect of biomass or aqueous extract of Desmodesmus subspicatus microalga and plant growth regulators (PGRs): 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA) on the in vitro propagation of C. warneri using protocorms as explants. Entire protocorms and sectioned transversely into two halves: apical and basal or longitudinal halves were grown on MS culture medium, with the concentration of macronutrients reduced by half (MSM/2) containing D. subspicatus biomass or extract and BA and/or NAA. The results indicated that the apical halves of protocorm were the best explants and the entire protocorms formed seedlings. The regeneration of protocorm-like bodies (PLBs) was more efficient with the addition of 1.5 g L−1 of microalgae biomass (92% and 4.7 PLBs) or 1.0 g L−1 of extract (100% and 3.3 PLBs) and 8 µM BA (89% and 5.2 PLBs). The biomass (1.0 g L−1) or extract (2.0 g L−1) also enabled PLBs regeneration from entire protocorms (70% and 4.0 PLBs, 50% and 3.2 PLBs, respectively). The plant acclimatization was recommended using coconut fiber and vermiculite (1:1, v/v) as substrate (survival rate 84% and greater fresh mass 0.895 g). In conclusion, a rapid and efficient protocol for the mass propagation of C. warneri was achieved using apical halves of protocorms, and MSM/2 medium supplementation with biomass or extract of D. subspicatus is an effective alternative to replace conventional PGRs.
Key message
Complete protocol for micropropagation of Cattleya warneri using PLBs regeneration was established. Apical halves of protocorms were the most responsive explant and Desmodesmus subspicatus microalga is alternative to replace PGRs.
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Acknowledgements
We grateful acknowledge to Dr. Rogerio Mamoru Suzuki for providing the seeds and photo of the Cattleya warneri flower used in this study.
Funding
The authors thank the “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES, Brazil) (Finance Code 001) and “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq, Brazil) for the Master´s scholarship to Quezia Rocha Navarro. Diego de Oliveira Corrêa acknowledges to CNPq for the postdoctoral scholarship (PDJ) (Grant No. 159590/2018-3). Miguel Daniel Noseda is Research Member of CNPq (Grant No. 306568/2018-7) and acknowledges CNPq for a Universal Call grant (Grant No. 462414/2014-0).
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QRN: methodology, validation, formal analysis, investigation, resources, data curation, writing—original draft, visualization. DdOC: conceptualization, methodology, validation, formal analysis, investigation, resources, writing—review & editing, supervision. AB: methodology, software, formal analysis, investigation, data curation, visualization. MDN: Conceptualization, methodology, resources, writing—review & editing, visualization. LLFR: conceptualization, methodology, validation, formal analysis, investigation, resources, writing—original draft, writing—review & editing, visualization, supervision, project administration.
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Navarro, Q.R., de Oliveira Corrêa, D., Behling, A. et al. Effect of microalga Desmodesmus subspicatus and plant growth regulators on the in vitro propagation of Cattleya warneri. Plant Cell Tiss Organ Cult 153, 77–89 (2023). https://doi.org/10.1007/s11240-022-02442-x
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DOI: https://doi.org/10.1007/s11240-022-02442-x